Protein phosphatase PP1-NIPP1 activates mesenchymal genes in HeLa cells.

Authors :: Van Dessel N
Boens S
Lesage B
Winkler C
Görnemann J
Van Eynde A
Bollen M
Source :: FEBS letters [FEBS Lett] 2015 May 22; Vol. 589 (12), pp. 1314-21. Date of Electronic Publication: 2015 Apr 20.
Publication Year :: 2015
Abstract: The deletion of the protein phosphatase-1 (PP1) regulator known as Nuclear Inhibitor of PP1 (NIPP1) is embryonic lethal during gastrulation, hinting at a key role of PP1-NIPP1 in lineage specification. Consistent with this notion we show here that a mild, stable overexpression of NIPP1 in HeLa cells caused a massive induction of genes of the mesenchymal lineage, in particular smooth/cardiac-muscle and matrix markers. This reprogramming was associated with the formation of actin-based stress fibers and retracting filopodia, and a reduced proliferation potential. The NIPP1-induced mesenchymal transition required functional substrate and PP1-binding domains, suggesting that it involves the selective dephosphorylation of substrates of PP1-NIPP1.<br /> (Copyright © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.)