Back to Search
Start Over
Combining microfluidics, optogenetics and calcium imaging to study neuronal communication in vitro.
- Source :
-
PloS one [PLoS One] 2015 Apr 22; Vol. 10 (4), pp. e0120680. Date of Electronic Publication: 2015 Apr 22 (Print Publication: 2015). - Publication Year :
- 2015
-
Abstract
- In this paper we report the combination of microfluidics, optogenetics and calcium imaging as a cheap and convenient platform to study synaptic communication between neuronal populations in vitro. We first show that Calcium Orange indicator is compatible in vitro with a commonly used Channelrhodopsine-2 (ChR2) variant, as standard calcium imaging conditions did not alter significantly the activity of transduced cultures of rodent primary neurons. A fast, robust and scalable process for micro-chip fabrication was developed in parallel to build micro-compartmented cultures. Coupling optical fibers to each micro-compartment allowed for the independent control of ChR2 activation in the different populations without crosstalk. By analyzing the post-stimuli activity across the different populations, we finally show how this platform can be used to evaluate quantitatively the effective connectivity between connected neuronal populations.
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 10
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 25901914
- Full Text :
- https://doi.org/10.1371/journal.pone.0120680