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Platelets convert peripheral blood circulating monocytes to regulatory cells via immunoglobulin G and activating-type Fcγ receptors.
- Source :
-
BMC immunology [BMC Immunol] 2015 Apr 21; Vol. 16, pp. 20. Date of Electronic Publication: 2015 Apr 21. - Publication Year :
- 2015
-
Abstract
- Background: Monocytes and macrophages produce interleukin (IL)-10, an immunoregulatory cytokine and a potent therapeutic tool for immune disorders. Augmentation of IL-10 production with a concomitant reduction of proinflammatory cytokines in macrophages in vitro is attained by doubly stimulating the cells with a toll-like receptor ligand and immunoglobulin (Ig)G immune complexes, a response known as that of regulatory (or alternatively activated/M2) macrophages. However, it has not been explored sufficiently how such a regulatory response could be exploited for anti-inflammation. Our objective is to find a potential way or condition for augmenting IL-10 by monocytes/macrophages in vivo and in vitro.<br />Results: We show that platelets, when they are opsonized with IgG, can convert human peripheral blood circulating monocytes to IL-10-producing regulatory monocytes in vitro and also in a murine in vivo model. Co-culturing of platelets and monocytes in the presence of anti-integrin IgG and a bacterial lipopolysaccharide augmented IL-10 production via a direct interaction between platelets and monocytes. This novel way of enhancing IL-10 was mediated by activating-type Fc receptors for IgG.<br />Conclusion: These findings indicate that the IgG-bound platelet-induced conversion of monocytes to regulatory cells might provide a novel strategy for controlling inflammation.
- Subjects :
- Antibodies, Blocking pharmacology
Blood Circulation
Blood Platelets drug effects
Cell Communication drug effects
Cell Differentiation drug effects
Cells, Cultured
Coculture Techniques
Humans
Immunomodulation
Integrins immunology
Integrins metabolism
Lipopolysaccharides metabolism
Monocytes drug effects
Blood Platelets immunology
Immunoglobulin G metabolism
Interleukin-10 metabolism
Monocytes immunology
Receptors, IgG metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2172
- Volume :
- 16
- Database :
- MEDLINE
- Journal :
- BMC immunology
- Publication Type :
- Academic Journal
- Accession number :
- 25896516
- Full Text :
- https://doi.org/10.1186/s12865-015-0086-z