Back to Search
Start Over
Standalone cohesin as a molecular shuttle in cellulosome assembly.
- Source :
-
FEBS letters [FEBS Lett] 2015 Jun 22; Vol. 589 (14), pp. 1569-76. Date of Electronic Publication: 2015 Apr 17. - Publication Year :
- 2015
-
Abstract
- The cellulolytic bacterium Ruminococcus flavefaciens of the herbivore rumen produces an elaborate cellulosome system, anchored to the bacterial cell wall via the covalently bound scaffoldin ScaE. Dockerin-bearing scaffoldins also bind to an autonomous cohesin of unknown function, called cohesin G (CohG). Here, we demonstrate that CohG binds to the scaffoldin-borne dockerin in opposite orientation on a distinct site, relative to that of ScaE. Based on these structural data, we propose that the complexed dockerin is still available to bind ScaE on the cell surface. CohG may thus serve as a molecular shuttle for delivery of scaffoldins to the bacterial cell surface.<br /> (Copyright © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Cell Cycle Proteins chemistry
Chromosomal Proteins, Non-Histone chemistry
Crystallography, X-Ray
Models, Molecular
Molecular Sequence Data
Sequence Homology, Amino Acid
Cohesins
Cell Cycle Proteins metabolism
Cellulosomes metabolism
Chromosomal Proteins, Non-Histone metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3468
- Volume :
- 589
- Issue :
- 14
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 25896019
- Full Text :
- https://doi.org/10.1016/j.febslet.2015.04.013