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Probiotic Lactobacillus casei Zhang reduces pro-inflammatory cytokine production and hepatic inflammation in a rat model of acute liver failure.
- Source :
-
European journal of nutrition [Eur J Nutr] 2016 Mar; Vol. 55 (2), pp. 821-831. Date of Electronic Publication: 2015 Apr 18. - Publication Year :
- 2016
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Abstract
- Purpose: In this study, we sought to find the effects and mechanisms of probiotic Lactobacillus casei Zhang (L. casei Zhang) on the pro-inflammatory cytokine production and hepatic inflammatory response in a rat model of acute liver failure induced by lipopolysaccharide (LPS) and d-galactosamine (GalN).<br />Methods: Male Wistar rats were orally administrated with or without L. casei Zhang for 30 days prior to challenge with LPS and GalN. Dexamethasone administrated group serving as a positive anti-inflammation control. Serum, intestinal and liver samples were collected 8 h after LPS/GalN challenge for histological, molecular and biochemical analysis.<br />Results: LPS/GalN challenge alone resulted in significantly increased production of endotoxin, tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β) and nitric oxide as compared to the normal control rats. Pretreatment with L. casei Zhang not only reduced these changes, but also attenuated hepatic inflammation as shown by improved histological assessment, decreased myeloperoxidase activity and reduced expression of IL-1β and inducible nitric oxide synthase in the liver. L. casei Zhang supplementation significantly inhibited LPS/GalN-triggered phosphorylation of ERK, JNK and p-38 MAPK, but increased the expression of TLR2, TLR9 and PPAR-γ. Moreover, L. casei Zhang treatment prevented intestinal injury and modulated the intestinal ecology by increasing the fecal Lactobacillus and Bifidobacterium levels.<br />Conclusions: Probiotic L. casei Zhang reduces LPS/GalN-induced pro-inflammatory cytokine and hepatic inflammation through modulating the TLR-MAPK-PPAR-γ signaling pathways and intestinal microbiota.
- Subjects :
- Acute Disease
Animals
Anti-Inflammatory Agents pharmacology
Bifidobacterium
Dexamethasone pharmacology
Disease Models, Animal
Extracellular Signal-Regulated MAP Kinases genetics
Extracellular Signal-Regulated MAP Kinases metabolism
Galactosamine adverse effects
Gastrointestinal Microbiome
JNK Mitogen-Activated Protein Kinases genetics
JNK Mitogen-Activated Protein Kinases metabolism
Lipopolysaccharides adverse effects
Liver metabolism
Male
Nitric Oxide blood
Nitric Oxide Synthase Type II genetics
Nitric Oxide Synthase Type II metabolism
PPAR gamma genetics
PPAR gamma metabolism
Rats
Rats, Wistar
Signal Transduction
Toll-Like Receptor 2 genetics
Toll-Like Receptor 2 metabolism
Toll-Like Receptor 9 genetics
Toll-Like Receptor 9 metabolism
Cytokines metabolism
Lacticaseibacillus casei
Liver microbiology
Liver Diseases therapy
Probiotics administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1436-6215
- Volume :
- 55
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- European journal of nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 25893720
- Full Text :
- https://doi.org/10.1007/s00394-015-0904-3