Back to Search
Start Over
Effects of Institut Georges Lopez-1 and Celsior preservation solutions on liver graft injury.
- Source :
-
World journal of gastroenterology [World J Gastroenterol] 2015 Apr 14; Vol. 21 (14), pp. 4159-68. - Publication Year :
- 2015
-
Abstract
- Aim: To compare Institut Georges Lopez (IGL-1) and Celsior preservation solutions for hepatic endothelium relaxation and liver cold ischemia reperfusion injury (IRI).<br />Methods: Two experimental models were used. In the first one, acetylcholine-induced endothelium-dependent relaxation (EDR) was measured in isolated ring preparations of rat hepatic arteries preserved or not in IGL-1 or Celsior solutions (24 h at 4 °C). To determine nitric oxide (NO) and cyclooxygenase EDR, hepatic arteries were incubated with L-NG-nitroarginine methyl ester (L-NAME), an inhibitor of endothelium nitric oxide synthase (eNOS), or with L-NAME plus indomethacin, an inhibitor of cyclooxygenase. In the second experiment, rat livers were cold-stored in IGL-1 or Celsior solutions for 24 h at 4 °C and then perfused "ex vivo" for 2 h at 37 °C. Liver injury was assessed by transaminase measurements, liver function by bile production and bromosulfophthalein clearance, oxidative stress by malondialdehyde levels and catalase activity and alterations in cell signaling pathways by pAkt, pAMPK, eNOS and MAPKs proteins level.<br />Results: After cold storage for 24 h with either Celsior or IGL-1, EDR was only slightly altered. In freshly isolated arteries, EDR was exclusively mediated by NO. However, cold-stored arteries showed NO- and COX-dependent relaxation. The decrease in NO-dependent relaxation after cold storage was significantly more marked with Celsior. The second study indicated that IGL-1 solution obtained better liver preservation and protection against IRI than Celsior. Liver injury was reduced, function was improved and there was less oxidative stress. IGL-1 solution activated Akt and AMPK, which was concomitant with increased eNOS expression and nitrite/nitrate levels. Furthermore, MAPKs kinases were regulated in livers preserved with IGL-1 solution since reductions in p-p38, p-ERK and p-JNK protein levels were observed.<br />Conclusion: IGL-1 solution preserved NO-dependent relaxation better than Celsior storage solution and enhanced liver graft preservation.
- Subjects :
- AMP-Activated Protein Kinases metabolism
Animals
Cold Ischemia
Cyclooxygenase Inhibitors pharmacology
Cytoprotection
Disaccharides pharmacology
Disease Models, Animal
Dose-Response Relationship, Drug
Electrolytes pharmacology
Endothelium, Vascular metabolism
Endothelium, Vascular physiopathology
Glutamates pharmacology
Glutathione pharmacology
Hepatic Artery metabolism
Hepatic Artery physiopathology
Histidine pharmacology
In Vitro Techniques
Liver blood supply
Liver metabolism
Male
Mannitol pharmacology
Mitogen-Activated Protein Kinases metabolism
Nitric Oxide metabolism
Nitric Oxide Synthase Type III antagonists & inhibitors
Nitric Oxide Synthase Type III metabolism
Oxidative Stress drug effects
Phosphorylation
Prostaglandin-Endoperoxide Synthases metabolism
Proto-Oncogene Proteins c-akt metabolism
Rats, Sprague-Dawley
Reperfusion Injury metabolism
Reperfusion Injury physiopathology
Signal Transduction drug effects
Time Factors
Vasodilator Agents pharmacology
Endothelium, Vascular drug effects
Hepatic Artery drug effects
Liver drug effects
Liver Transplantation methods
Organ Preservation methods
Organ Preservation Solutions pharmacology
Reperfusion Injury prevention & control
Vasodilation drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 2219-2840
- Volume :
- 21
- Issue :
- 14
- Database :
- MEDLINE
- Journal :
- World journal of gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 25892865
- Full Text :
- https://doi.org/10.3748/wjg.v21.i14.4159