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ACTN4 and the pathways associated with cell motility and adhesion contribute to the process of lung cancer metastasis to the brain.
- Source :
-
BMC cancer [BMC Cancer] 2015 Apr 12; Vol. 15, pp. 277. Date of Electronic Publication: 2015 Apr 12. - Publication Year :
- 2015
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Abstract
- Background: The aim of this study was to identify critical gene pathways that are associated with lung cancer metastasis to the brain.<br />Methods: The RNA-Seq approach was used to establish the expression profiles of a primary lung cancer, adjacent benign tissue, and metastatic brain tumor from a single patient. The expression profiles of these three types of tissues were compared to define differentially expressed genes, followed by serial-cluster analysis, gene ontology analysis, pathway analysis, and knowledge-driven network analysis. Reverse transcription-polymerase chain reaction (RT-PCR) was used to validate the expression of essential candidate genes in tissues from ten additional patients.<br />Results: Differential gene expression among these three types of tissues was classified into multiple clusters according to the patterns of their alterations. Further bioinformatic analysis of these expression profile data showed that the network of the signal transduction pathways related to actin cytoskeleton reorganization, cell migration, and adhesion was associated with lung cancer metastasis to the brain. The expression of ACTN4 (actinin, alpha 4), a cytoskeleton protein gene essential for cytoskeleton organization and cell motility, was significantly elevated in the metastatic brain tumor but not in the primary lung cancer tissue.<br />Conclusions: The signaling pathways involved in the regulation of cytoskeleton reorganization, cell motility, and focal adhesion play a role in the process of lung cancer metastasis to the brain. The contribution of ACTN4 to the process of lung cancer metastasis to the brain could be mainly through regulation of actin cytoskeleton reorganization, cell motility, and focal adhesion.
- Subjects :
- Actinin biosynthesis
Brain Neoplasms pathology
Brain Neoplasms secondary
Cell Adhesion genetics
Cell Movement genetics
Female
Gene Expression Regulation, Neoplastic
Gene Regulatory Networks
High-Throughput Nucleotide Sequencing
Humans
Lung Neoplasms pathology
Middle Aged
Neoplasm Metastasis
Signal Transduction genetics
Actinin genetics
Brain Neoplasms genetics
Cytoskeleton genetics
Lung Neoplasms genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2407
- Volume :
- 15
- Database :
- MEDLINE
- Journal :
- BMC cancer
- Publication Type :
- Academic Journal
- Accession number :
- 25885339
- Full Text :
- https://doi.org/10.1186/s12885-015-1295-9