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Adrenergic antagonists restrict replication of Legionella.
- Source :
-
Microbiology (Reading, England) [Microbiology (Reading)] 2015 Jul; Vol. 161 (7), pp. 1392-406. Date of Electronic Publication: 2015 Apr 14. - Publication Year :
- 2015
-
Abstract
- Legionella pneumophila is a facultative intracellular bacterium, which upon inhalation can cause a potentially fatal pneumonia termed Legionnaires' disease. The opportunistic pathogen grows in environmental amoebae and mammalian macrophages within a unique membrane-bound compartment, the 'Legionella-containing vacuole'. Bacteria are exposed to many environmental cues including small signalling molecules from eukaryotic cells. A number of pathogenic bacteria sense and respond to catecholamine hormones, such as adrenalin and noradrenalin, a process mediated via the QseBC two-component system in some bacteria. In this study, we examined the effect of adrenergic compounds on L. pneumophila, and discovered that the adrenergic receptor antagonists benoxathian, naftopidil, propranolol and labetalol, as well as the QseC sensor kinase inhibitor LED209, reduced the growth of L. pneumophila in broth or amoebae, while replication in macrophages was enhanced. Growth restriction was common to members of the genus Legionella and Mycobacterium, and was observed for L. pneumophila in the replicative but not stationary phase of the biphasic life cycle. Deletion of the L. pneumophila qseBC genes indicated that growth inhibition by adrenergics or LED209 is mediated only to a minor extent by this two-component system, implying the presence of other adrenergic sensing systems. This study identifies adrenergic molecules as novel inhibitors of extra- and intracellular growth of Legionella and reveals LED209 as a potential lead compound to combat infections with Legionella or Mycobacterium spp.
- Subjects :
- Acanthamoeba castellanii drug effects
Acanthamoeba castellanii microbiology
Animals
Cell Line
Macrophages drug effects
Macrophages microbiology
Mice
Mycobacterium drug effects
Mycobacterium growth & development
Adrenergic Antagonists metabolism
Anti-Bacterial Agents metabolism
Legionella pneumophila drug effects
Legionella pneumophila growth & development
Subjects
Details
- Language :
- English
- ISSN :
- 1465-2080
- Volume :
- 161
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Microbiology (Reading, England)
- Publication Type :
- Academic Journal
- Accession number :
- 25873585
- Full Text :
- https://doi.org/10.1099/mic.0.000094