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In vitro and in vivo Bone-Forming Activity of Saururus chinensis Extract.
- Source :
-
Phytotherapy research : PTR [Phytother Res] 2015 Jul; Vol. 29 (7), pp. 1073-80. Date of Electronic Publication: 2015 Apr 14. - Publication Year :
- 2015
-
Abstract
- Bone is maintained by osteoclast-mediated resorption and osteoblast-mediated formation. Recently, anti-osteoporotic activity of Saururus chinensis extract (SCE) and anti-osteoclastogenic activity of its components have been reported, but the effect of SCE on bone formation has not been studied well. Therefore, in this study, we investigated whether Saururus chinensis SCE exhibits in vitro osteogenic and in vivo bone-forming activity. extract strongly enhanced the bone morphogenetic protein (BMP)-2-stimulated induction of alkaline phosphatase, an early phase biomarker of osteoblast differentiation, in bi-potential mesenchymal progenitor C2C12 cells. In vitro osteogenic activity of SCE was accompanied by enhanced expression of BMP-2, BMP-4, BMP-7 and BMP-9 mRNA. In addition, a pharmacological inhibition study suggested the involvement of p38 activation in the osteogenic action of SCE. Moreover, the BMP dependency and the involvement of p38 activation in the osteogenic action of SCE were confirmed by the treatment of noggin, an antagonist of BMP. Saururus chinensis extract also exhibited to induce runt-related transcription factor 2 activation at the high concentration. Furthermore, the in vivo osteogenic activity of SCE was confirmed in zebrafish and mouse calvarial bone formation models, suggesting the possibility of its use for bone formation. In conclusion, we suggested that in vivo anti-osteoporotic activity of SCE could be because of its dual action in bone, anti-osteoclastogenic and anabolic activity.<br /> (Copyright © 2015 John Wiley & Sons, Ltd.)
- Subjects :
- Alkaline Phosphatase metabolism
Animals
Cell Differentiation drug effects
Cell Line
Core Binding Factor Alpha 1 Subunit metabolism
Mice
Mice, Inbred ICR
Zebrafish
p38 Mitogen-Activated Protein Kinases metabolism
Bone Morphogenetic Proteins metabolism
Osteoblasts drug effects
Osteogenesis drug effects
Plant Extracts pharmacology
Saururaceae chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1099-1573
- Volume :
- 29
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Phytotherapy research : PTR
- Publication Type :
- Academic Journal
- Accession number :
- 25869918
- Full Text :
- https://doi.org/10.1002/ptr.5349