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Surgical method for virally mediated gene delivery to the mouse inner ear through the round window membrane.
- Source :
-
Journal of visualized experiments : JoVE [J Vis Exp] 2015 Mar 16 (97). Date of Electronic Publication: 2015 Mar 16. - Publication Year :
- 2015
-
Abstract
- Gene therapy, used to achieve functional recovery from sensorineural deafness, promises to grant better understanding of the underlying molecular and genetic mechanisms that contribute to hearing loss. Introduction of vectors into the inner ear must be done in a way that widely distributes the agent throughout the cochlea while minimizing injury to the existing structures. This manuscript describes a post-auricular surgical approach that can be used for mouse cochlear therapy using molecular, pharmacologic, and viral delivery to mice postnatal day 10 and older via the round window membrane (RWM). This surgical approach enables rapid and direct delivery into the scala tympani while minimizing blood loss and avoiding animal mortality. This technique involves negligible or no damage to essential structures of the inner and middle ear as well as neck muscles while wholly preserving hearing. To demonstrate the efficacy of this surgical technique, the vesicular glutamate transporter 3 knockout (VGLUT3 KO) mice will be used as an example of a mouse model of congenital deafness that recovers hearing after delivery of VGLUT3 to the inner ear using an adeno-associated virus (AAV-1).
- Subjects :
- Amino Acid Transport Systems, Acidic administration & dosage
Amino Acid Transport Systems, Acidic genetics
Animals
Deafness congenital
Deafness genetics
Deafness therapy
Female
Genetic Therapy methods
Genetic Vectors genetics
Male
Mice
Mice, Knockout
Dependovirus genetics
Gene Transfer Techniques
Genetic Vectors administration & dosage
Round Window, Ear surgery
Subjects
Details
- Language :
- English
- ISSN :
- 1940-087X
- Issue :
- 97
- Database :
- MEDLINE
- Journal :
- Journal of visualized experiments : JoVE
- Publication Type :
- Academic Journal
- Accession number :
- 25867531
- Full Text :
- https://doi.org/10.3791/52187