Back to Search
Start Over
Impedimetric DNA-biosensor for the study of dopamine induces DNA damage and investigation of inhibitory and repair effects of some antioxidants.
- Source :
-
Bioelectrochemistry (Amsterdam, Netherlands) [Bioelectrochemistry] 2015 Aug; Vol. 104, pp. 71-8. Date of Electronic Publication: 2015 Apr 01. - Publication Year :
- 2015
-
Abstract
- A simple and inexpensive methodology was used to develop a new method in order to inspect the DNA damage due to dopamine and some ionic metals. In addition, the inhibitory and repair effects of some antioxidant such as glutathione and ascorbic acid were studied and compared with each other using electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). In this work a pencil graphite electrode (PGE) was modified with multiwall carbon nanotubes (MWCNTs) and chitosan (CHIT), then it was decorated with a ds-DNA (ds-DNA/CHIT-MWCNTs/PGE). Due to interaction of ds-DNA and the damaging agents (dopamine+metallic ions), electrochemical and spectroscopy properties of ds-DNA at the surface of the modified electrode was changed, and these changes are followed with EIS and DPV methods. Our study showed that dopamine, Cu(II) and Fe(III) alone could not destroy DNA, but dopamine + Cu(II) and dopamine + Fe(III) can damage DNA. In addition, the ability of dopamine-Cu(II) was greater than dopamine-Fe(III). Moreover, some antioxidant such as glutathione and ascorbic acid can overcome and/or minimize the influence of these damaging interactions.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Subjects :
- Ascorbic Acid pharmacology
Chitosan chemistry
Copper chemistry
DNA chemistry
Dopamine pharmacology
Electrodes
Glutathione pharmacology
Graphite chemistry
Iron chemistry
Nanotubes, Carbon chemistry
Oxidative Stress drug effects
Surface Properties
Antioxidants pharmacology
Biosensing Techniques methods
DNA genetics
DNA Damage
DNA Repair drug effects
Dielectric Spectroscopy
Dopamine analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1878-562X
- Volume :
- 104
- Database :
- MEDLINE
- Journal :
- Bioelectrochemistry (Amsterdam, Netherlands)
- Publication Type :
- Academic Journal
- Accession number :
- 25866909
- Full Text :
- https://doi.org/10.1016/j.bioelechem.2015.03.008