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Induction of survivin inhibition, G₂/M cell cycle arrest and autophagic on cell death in human malignant glioblastoma cells.
- Source :
-
The Chinese journal of physiology [Chin J Physiol] 2015 Apr 30; Vol. 58 (2), pp. 95-103. - Publication Year :
- 2015
-
Abstract
- Chemotherapy efficacy is limited by intrinsic and acquired resistance in glioblastoma (GBM); hence, novel tactics are crucial. Survivin has been demonstrated as a key resistant factor in GBM because of its function in inhibiting apoptosis, regulating autophagy, and in promoting G₂/M cell cycle transition. Parthenolide has been reported to be an effective antitumor agent in a variety of tumor cells and decreases survivin level in leukemia cells. But the effect of parthenolide on survivin and the cell death process in GBM is still unknown. The aim of this study was to examine whether parthenolide had the potential to inhibit cell proliferation in the GBM cell line U373. The parthenolide-induced effects in relation to survivin suppression and cell death were further investigated. Our results showed that parthenolide substantially inhibited cell viability with IC₅₀ values of approximate 16 μM. Treatment with parthenolide at the dose of 16 μM led to considerable downregulation of survivin, G₂/M cell cycle arrest and Chk2 upregulation in cells. Parthenolide induced apoptosis in only a few cells and a slight increase in activated caspases 3 levels. By contrast, parthenolide induced a significant increase of intracellular autophagosomes and the expression of autophagy related proteins, including ULK1 and LC3 I/LC3 II, in the treated cells. These results suggested that parthenolide induced survivin inhibition, G₂/M cell cycle arrest, and triggered cell death through autophagic cell death in the GBM cell line.
- Subjects :
- Antineoplastic Agents administration & dosage
Apoptosis drug effects
Autophagy drug effects
Cell Line, Tumor
Dose-Response Relationship, Drug
Humans
Inhibitor of Apoptosis Proteins antagonists & inhibitors
Survivin
Treatment Outcome
G2 Phase Cell Cycle Checkpoints drug effects
Glioblastoma drug therapy
Glioblastoma pathology
Inhibitor of Apoptosis Proteins metabolism
M Phase Cell Cycle Checkpoints drug effects
Sesquiterpenes administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 0304-4920
- Volume :
- 58
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The Chinese journal of physiology
- Publication Type :
- Academic Journal
- Accession number :
- 25858470
- Full Text :
- https://doi.org/10.4077/CJP.2015.BAC267