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[Saponin 6 of Anemone Taipaiensis inhibits proliferation and induces apoptosis of U87 MG cells].

Authors :
Ji C
Cheng G
Tang H
Zhang Y
Hu Y
Zheng M
Fei Z
Source :
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi] 2015 Apr; Vol. 31 (4), pp. 484-6.
Publication Year :
2015

Abstract

Objective: To investigate the effect of saponin 6 of Anemone Taipaiensis on the proliferation of human U87 MG glioma cells and the possible mechanism.<br />Methods: U87 MG cells were treated with different concentrations of saponin 6 (0.0, 1.6, 3.2, 6.4, 12.8 μg/mL) for 24 hours or 48 hours. Cell viability was measured by MTT assay; the apoptosis rate was detected by flow cytometry combined with annexin V-FITC /PI staining; Western blotting was applied to determine the protein level of activated caspase-3.<br />Results: Compared with control groups, saponin 6 significantly inhibited U87 MG cell proliferation in a time- and dose-depended manner. Apoptosis rate of U87 MG cells and the expression of activated caspase-3 were raised with the increasing concentration of saponin 6.<br />Conclusion: Saponin 6 of Anemone Taipaiensis could depress cell proliferation in a dose-depended manner, increase the expression of activated caspase-3 and promote apoptosis in U87 MG cells.

Details

Language :
Chinese
ISSN :
1007-8738
Volume :
31
Issue :
4
Database :
MEDLINE
Journal :
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Publication Type :
Academic Journal
Accession number :
25854568