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Effect of varying doses of tamoxifen on ovarian histopathology, serum VEGF, and endothelin 1 levels in ovarian hyperstimulation syndrome: an experimental study.
- Source :
-
Drug design, development and therapy [Drug Des Devel Ther] 2015 Mar 24; Vol. 9, pp. 1761-6. Date of Electronic Publication: 2015 Mar 24 (Print Publication: 2015). - Publication Year :
- 2015
-
Abstract
- Objective: To examine the effects of low-to-high doses of tamoxifen on ovarian histopathology, serum VEGF, and endothelin 1 levels in ovarian hyperstimulation syndrome (OHSS) in an experimental setting.<br />Materials and Methods: A total of 20 female Wistar albino rats, 22 days of age, were randomly divided into four groups. Follicle-stimulating hormone 10 IU was administered subcutaneously in 15 rats on 4 consecutive days, with OHSS induction on day 5 by 30 IU of human chorionic gonadotropin. Group 1 (n=5) comprised 35-day-old control rats, group 2 (n=5) 35-day-old OHSS rats, group 3 (n=5) 27-day-old OHSS rats receiving 1 mg/kg of oral tamoxifen for 7 days, group 4 (n=5) 27-day-old OHSS rats receiving 3 mg/kg of oral tamoxifen for 7 days. All rats were decapitated on day 35. Serum VEGF, endothelin 1, and ovarian follicular reserve were assessed in all rats. Kruskal-Wallis variance analysis and the Mann-Whitney U-test were used for statistical comparisons. A Bonferroni correction was performed to control the inflation of significance, with a significance level set at a P-value of less than 0.025.<br />Results: Despite higher serum VEGF, endothelin 1, follicular reserve, and angiogenesis and fibrosis of the corpus luteum in the OHSS group compared to controls, these differences were not significant (P>0.025, Mann-Whitney U-test). There was a significant reduction in the ovarian follicular reserve in tamoxifen groups compared to controls (P<0.025, Mann-Whitney U-test), while angiogenesis of the corpus luteum, number of atretic follicles, fibrosis, and serum VEGF were significantly higher in rats receiving tamoxifen (P<0.025, Mann-Whitney U-test). Also, significantly lower follicular reserve and fibrosis were observed among rats in the low-dose tamoxifen group in comparison with rats in the high-dose tamoxifen group (P<0.025, Mann-Whitney U-test). No groups had a significant change in endothelin 1 levels (P>0.025, Mann-Whitney U-test).<br />Conclusion: Tamoxifen 1 g and 3 g resulted in a dose-dependent increase in VEGF and endothelin 1 levels, and ovarian follicle reserves were significantly reduced in our experimental model.
- Subjects :
- Administration, Oral
Animals
Disease Models, Animal
Dose-Response Relationship, Drug
Female
Gonadotropins administration & dosage
Gonadotropins pharmacology
Humans
Ovarian Hyperstimulation Syndrome chemically induced
Ovarian Hyperstimulation Syndrome drug therapy
Rats
Rats, Wistar
Tamoxifen therapeutic use
Endothelin-1 blood
Ovarian Hyperstimulation Syndrome blood
Ovarian Hyperstimulation Syndrome pathology
Tamoxifen administration & dosage
Tamoxifen pharmacology
Vascular Endothelial Growth Factor A blood
Subjects
Details
- Language :
- English
- ISSN :
- 1177-8881
- Volume :
- 9
- Database :
- MEDLINE
- Journal :
- Drug design, development and therapy
- Publication Type :
- Academic Journal
- Accession number :
- 25848212
- Full Text :
- https://doi.org/10.2147/DDDT.S75266