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Evidence of a target resistance to antivitamin K rodenticides in the roof rat Rattus rattus: identification and characterisation of a novel Y25F mutation in the Vkorc1 gene.
- Source :
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Pest management science [Pest Manag Sci] 2016 Mar; Vol. 72 (3), pp. 544-50. Date of Electronic Publication: 2015 May 26. - Publication Year :
- 2016
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Abstract
- Background: In spite of intensive use of bromadiolone, rodent control was inefficient on a farm infested by rats in Zaragoza, Spain. While metabolic resistance was previously described in this rodent species, the observation of a target resistance to antivitamin K rodenticides had been poorly documented in Rattus rattus.<br />Results: From rats trapped on the farm, cytochrome b and Vkorc1 genes were amplified by PCR and sequenced in order to identify species and detect potential Vkorc1 mutations. VKORC1-deduced amino acid sequences were thus expressed in Pichia pastoris, and inhibition constants towards various rodenticides were determined. The ten rats trapped on the farm were all identified as R. rattus. They were found to be homozygous for the g.74A>T nucleotide replacement in exon 1 of the Vkorc1 gene, leading to p.Y25F mutation. This mutation led to increased apparent inhibition constants towards various rodenticides, probably caused by a partial loss of helical structure of TM4.<br />Conclusion: The p.Y25F mutation detected in the Vkorc1 gene in R. rattus trapped on the Spanish farm is associated with the resistance phenotype to bromadiolone that has been observed. It is the first evidence of target resistance to antivitamin K anticoagulants in R. rattus.<br /> (© 2015 Society of Chemical Industry.)
- Subjects :
- Amino Acid Sequence
Animals
Anticoagulants pharmacology
Rats metabolism
Rodent Control
Sequence Alignment
Spain
Vitamin K metabolism
Vitamin K Epoxide Reductases metabolism
4-Hydroxycoumarins metabolism
4-Hydroxycoumarins pharmacology
Drug Resistance genetics
Indenes metabolism
Mutation
Rats genetics
Rodenticides pharmacology
Vitamin K antagonists & inhibitors
Vitamin K Epoxide Reductases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1526-4998
- Volume :
- 72
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Pest management science
- Publication Type :
- Academic Journal
- Accession number :
- 25847836
- Full Text :
- https://doi.org/10.1002/ps.4020