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Prime-boost vaccination strategy with bacillus Calmette-Guérin (BCG) and liposomized alpha-crystalline protein 1 reinvigorates BCG potency.
- Source :
-
Clinical and experimental immunology [Clin Exp Immunol] 2015 Aug; Vol. 181 (2), pp. 286-96. Date of Electronic Publication: 2015 Jun 03. - Publication Year :
- 2015
-
Abstract
- Bacillus Calmette-Guérin (BCG) remains the only available and most widely administered vaccine against Mycobacterium tuberculosis (Mtb), yet it fails to protect vaccinated individuals either from primary infection or reactivation of latent tuberculosis (TB). Despite BCG's variable efficacy against TB, the fact remains that BCG imparts protection in children against the disease, indicating that BCG possesses a wide protective antigenic repertoire. However, its failure to impart protection in adulthood can be linked to its failure to generate long-lived memory response and elicitation of an inadequate immune response against latency-associated antigens. Therefore, to improve the protective efficacy of BCG, a novel vaccination strategy is required. Consequently, in the present study, we have exploited the vaccination potential of liposomized α-crystalline 1 (Acr1L), a latency-associated antigen to induce enduring protective immunity against Mtb in BCG-primed animals. It is noteworthy that an increase in the multi-functional [interferon (IFN)-γ(hi) /tumour necrosis factor (TNF)-α(hi) ] CD4 and CD8 T cells were observed in BCG-primed and Acr1L-boosted (BCG-Acr1L) animals, compared to BCG alone. Further, substantial expansion of both central memory (CD44(hi) /CD62L(hi) ) and effector memory (CD44(hi) /CD62L(lo) ) populations of CD4 and CD8 T cells was noted. Importantly, BCG-Acr1L exhibited significantly better protection than BCG, as evidenced by a reduction in the bacterial burden and histopathological data of the lungs. In essence, BCG-Acr1L could be a potent future vaccination strategy to reinvigorate BCG potency.<br /> (© 2015 Institute of Microbial Technology.)
- Subjects :
- Animals
BCG Vaccine administration & dosage
BCG Vaccine genetics
Bacterial Load drug effects
Bacterial Proteins genetics
CD4-Positive T-Lymphocytes drug effects
CD4-Positive T-Lymphocytes immunology
CD4-Positive T-Lymphocytes microbiology
CD4-Positive T-Lymphocytes pathology
CD8-Positive T-Lymphocytes drug effects
CD8-Positive T-Lymphocytes immunology
CD8-Positive T-Lymphocytes microbiology
CD8-Positive T-Lymphocytes pathology
Female
Hyaluronan Receptors genetics
Hyaluronan Receptors immunology
Immunologic Memory drug effects
Immunophenotyping
Interferon-gamma biosynthesis
Interferon-gamma immunology
L-Selectin genetics
L-Selectin immunology
Latent Tuberculosis immunology
Latent Tuberculosis microbiology
Latent Tuberculosis pathology
Liposomes chemistry
Liposomes immunology
Lung drug effects
Lung immunology
Lung microbiology
Lung pathology
Mice
Mycobacterium bovis chemistry
Mycobacterium bovis immunology
Mycobacterium tuberculosis growth & development
Mycobacterium tuberculosis immunology
Recombinant Fusion Proteins administration & dosage
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins immunology
Tumor Necrosis Factor-alpha biosynthesis
Tumor Necrosis Factor-alpha immunology
alpha-Crystallins genetics
BCG Vaccine immunology
Bacterial Proteins immunology
Immunization, Secondary
Latent Tuberculosis prevention & control
Mycobacterium tuberculosis drug effects
alpha-Crystallins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1365-2249
- Volume :
- 181
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Clinical and experimental immunology
- Publication Type :
- Academic Journal
- Accession number :
- 25845290
- Full Text :
- https://doi.org/10.1111/cei.12634