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Prauserella endophytica sp. nov., an endophytic actinobacterium isolated from Tamarix taklamakanensis.
- Source :
-
Antonie van Leeuwenhoek [Antonie Van Leeuwenhoek] 2015 Jun; Vol. 107 (6), pp. 1401-9. Date of Electronic Publication: 2015 Apr 03. - Publication Year :
- 2015
-
Abstract
- A novel endophytic actinobacterium, designated strain SP28S-3(T), was isolated from a surface-sterilized stem of Tamarix taklamakanensis collected from the southern edge of Taklamakan desert, Xinjiang, China. Strain SP28S-3(T) was found to show chemotaxonomic and morphological properties consistent with its classification in the genus Prauserella. The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphoglycolipid, phosphatidylcholine, phosphatidylinositol, a glycolipid, an aminolipid and unidentified phospholipids. The major fatty acids (>10 %) were identified as iso-C16:0 and C16:0. The genomic DNA G+C content was determined to be 69.7 mol%. Phylogenetic analysis of strain SP28S-3(T) clearly showed that the strain had the highest similarity of 16S rRNA gene sequence with Prauserella coralliicola SCSIO 11529(T) (99.9 %), followed by Prauserella marina DSM 45268(T) (97.0 %) and is affiliated with the genus Prauserella. The low level (47.8 ± 5.5 %) of DNA-DNA relatedness between strain SP28S-3(T) and P. coralliicola SCSIO 11529(T) combined with other polyphasic taxonomic evidence clearly support the conclusion that strain SP28S-3(T) represents a novel Prauserella species, for which the name Prauserella endophytica sp. nov. is proposed. The type strain is SP28S-3(T) (=DSM 46655(T) = CGMCC 4.7182 (T)).
- Subjects :
- Actinobacteria genetics
Actinobacteria physiology
Bacterial Typing Techniques
Base Composition
China
Cluster Analysis
Cytosol chemistry
DNA, Bacterial chemistry
DNA, Bacterial genetics
DNA, Ribosomal chemistry
DNA, Ribosomal genetics
Endophytes genetics
Endophytes physiology
Fatty Acids analysis
Glycolipids analysis
Molecular Sequence Data
Nucleic Acid Hybridization
Phospholipids analysis
Phylogeny
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Actinobacteria classification
Actinobacteria isolation & purification
Endophytes classification
Endophytes isolation & purification
Tamaricaceae microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1572-9699
- Volume :
- 107
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Antonie van Leeuwenhoek
- Publication Type :
- Academic Journal
- Accession number :
- 25832628
- Full Text :
- https://doi.org/10.1007/s10482-015-0435-1