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Progression of matrixin and cardiokine expression patterns in an ovine model of heart failure and recovery.
- Source :
-
International journal of cardiology [Int J Cardiol] 2015; Vol. 186, pp. 77-89. Date of Electronic Publication: 2015 Mar 17. - Publication Year :
- 2015
-
Abstract
- Background: The molecular mechanisms underlying the geometrical changes of the left ventricle during the progression to heart failure and recovery are not well defined.<br />Objective: Here we investigate the involvement of matrixins and cardiokines in an ovine model of pressure-induced left ventricular failure (LVF).<br />Methods: Fifteen sheep underwent supracoronary aortic banding with an inflatable cuff. A controlled and progressive increase of LV pressure was monitored echocardiographically. Endomyocardial biopsies were collected throughout the development of LVF and subsequent recovery after pressure unloading.<br />Results: Thirteen sheep developed LVF with a subsequent recovery. Peak left ventricular hypertrophy (LVH) and dilatation (LVD) occurred at 31.5 ± 1.6 weeks and 102.7 ± 2.2 weeks post-banding respectively, with an increase in LV internal diameter in diastole (LVIDd 5.11 ± 0.12 compared to the control 3.37 ± 0.07 cm, p<0.001), with preserved LV ejection fraction (LVEF). Reduced LVEF became evident 116.5 ± 2.7 weeks post-banding. Clinical and echocardiographic improvements were observed following deflation of the aortic banding cuff. By 138.1 ± 3.1 weeks cardiac performance recovered with restoration of LVEF. Significant changes in the expression of matrix metalloproteinases (MMP)-1, -2, -3, vascular endothelial cell growth factor (VEGF), fibroblast growth factor (FGF)-2, interferon (INF)-α-2 and soluble CD40 ligand (sCD40L) were observed throughout the progression to failure and recovery.<br />Conclusions: We used an ovine model to study reversible LV remodelling without interruption and found significant changes in matrixin and cardiokine expression during LV progression to failure and recovery.<br /> (Copyright © 2015. Published by Elsevier Ireland Ltd.)
- Subjects :
- Animals
Cytokines biosynthesis
Disease Models, Animal
Disease Progression
Heart Failure metabolism
Heart Failure physiopathology
Immunoblotting
Matrix Metalloproteinases biosynthesis
Real-Time Polymerase Chain Reaction
Sheep
Sheep, Domestic
Cytokines genetics
Gene Expression Regulation
Heart Failure genetics
Matrix Metalloproteinases genetics
RNA genetics
Recovery of Function physiology
Ventricular Remodeling
Subjects
Details
- Language :
- English
- ISSN :
- 1874-1754
- Volume :
- 186
- Database :
- MEDLINE
- Journal :
- International journal of cardiology
- Publication Type :
- Academic Journal
- Accession number :
- 25814349
- Full Text :
- https://doi.org/10.1016/j.ijcard.2015.03.156