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The marine metabolite SZ-685C induces apoptosis in primary human nonfunctioning pituitary adenoma cells by inhibition of the Akt pathway in vitro.
- Source :
-
Marine drugs [Mar Drugs] 2015 Mar 23; Vol. 13 (3), pp. 1569-80. Date of Electronic Publication: 2015 Mar 23. - Publication Year :
- 2015
-
Abstract
- Nonfunctioning pituitary adenoma (NFPA) is one of the most common types of pituitary adenoma. The marine anthraquinone derivative SZ-685C has been isolated from the secondary metabolites of the mangrove endophytic fungus Halorosellinia sp. (No. 1403) which is found in the South China Sea. Recent research has shown that SZ-685C possesses anticancer and tumor suppressive effects. The tetrazolium-based colorimetric assay (MTT assay) to investigate the different effect of the marine compound SZ-685C on the proliferation of primary human NFPA cells, rat normal pituitary cells (RPCs) and rat prolactinoma MMQ cell lines. Hoechst 33342 dye/propidium iodide (PI) double staining and fluorescein isothiocyanate-conjugated Annexin V/PI (Annexin V-FITC/PI) apoptosis assays detected an enhanced rate of apoptosis in cells treated with SZ-685C. Enhanced expression levels of caspase 3 and phosphate and tensin homolog (PTEN) were determined by Western blotting. Notably, the protein expression levels of Akt were decreased when the primary human NFPA cells were treated with SZ-685C. Here, we show that SZ-685C induces apoptosis of human NFPA cells through inhibition of the Akt pathway in vitro. The understanding of apoptosis has provided the basis for novel targeted therapies that can induce death in cancer cells or sensitize them to established cytotoxic agents and radiation therapy.
- Subjects :
- Adenoma drug therapy
Adenoma pathology
Animals
Anthraquinones isolation & purification
Antineoplastic Agents isolation & purification
Ascomycota metabolism
Cell Line, Tumor
Cell Proliferation drug effects
China
Humans
Pituitary Neoplasms pathology
Prolactinoma drug therapy
Prolactinoma pathology
Proto-Oncogene Proteins c-akt antagonists & inhibitors
Rats
Secondary Metabolism
Anthraquinones pharmacology
Antineoplastic Agents pharmacology
Apoptosis drug effects
Pituitary Neoplasms drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1660-3397
- Volume :
- 13
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Marine drugs
- Publication Type :
- Academic Journal
- Accession number :
- 25806467
- Full Text :
- https://doi.org/10.3390/md13031569