Utilization of a reporter system based on the blue pigment indigoidine biosynthetic gene bpsA for detection of promoter activity and deletion of genes in Streptomyces.

Authors :: Knirschova R
Novakova R
Mingyar E
Bekeova C
Homerova D
Kormanec J
Source :: Journal of microbiological methods [J Microbiol Methods] 2015 Jun; Vol. 113, pp. 1-3. Date of Electronic Publication: 2015 Mar 20.
Publication Year :: 2015
Abstract: The integrative promoter-probe plasmid pBPSA1 was constructed using a promoterless Streptomyces aureofaciens CCM3239 bpsA gene encoding a non-ribosomal peptide synthase for the biosynthesis of a blue pigment, indigoidine. bpsA was also used to prepare pAMR4 plasmid for the deletion of genes in Streptomyces with facile identification of double crossover recombination.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)

Subjects :: Peptide Synthases genetics
Plasmids
Recombination, Genetic
Streptomyces aureofaciens enzymology
Genes, Bacterial
Genes, Reporter
Piperidones metabolism
Promoter Regions, Genetic
Sequence Deletion
Streptomyces aureofaciens genetics

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