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A novel laccase from fresh fruiting bodies of the wild medicinal mushroom Tricholoma matsutake.
- Source :
-
Acta biochimica Polonica [Acta Biochim Pol] 2015; Vol. 62 (1), pp. 35-40. Date of Electronic Publication: 2015 Mar 18. - Publication Year :
- 2015
-
Abstract
- The knowledge about biological activities of constituents from medicinal mushrooms belonging to the genus Tricholoma is limited. A 59-kDa laccase has now been purified from fresh fruiting bodies of the mushroom Tricholoma matsutake. The purification protocol entailed ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on CM-cellulose, affinity chromatography on ConA-Sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75. Of the various affinity and ion exchange chromatographic media employed, the laccase bound only on Con A-Sepharose. The activity of the laccase did not undergo major changes over the temperature range 20-80°C. However, all activity vanished following exposure to 100°C for 10 minutes. The enzyme activity varied only slightly over the pH range 3-5, with the optimal pH of 5, but exhibited a precipitous decline when the pH was increased to 6, and was undetectable at pH 8 and 9. The laccase showed activity in the decolorization of azo dyes without a mediator. Its N-terminal sequence demonstrated only slight resemblance to those of other mushroom laccases. The newly described laccase is distinctive from the previously isolated Tricholoma mushroom laccases in a number of aspects.
- Subjects :
- Amino Acid Sequence
Chromatography, Liquid methods
Color
Electrophoresis, Polyacrylamide Gel
HIV Reverse Transcriptase antagonists & inhibitors
Hydrogen-Ion Concentration
Laccase chemistry
Laccase pharmacology
Molecular Weight
Reverse Transcriptase Inhibitors pharmacology
Temperature
Fruiting Bodies, Fungal enzymology
Laccase isolation & purification
Tricholoma enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1734-154X
- Volume :
- 62
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Acta biochimica Polonica
- Publication Type :
- Academic Journal
- Accession number :
- 25781157
- Full Text :
- https://doi.org/10.18388/abp.2014_713