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MTDH/AEG-1-based DNA vaccine suppresses metastasis and enhances chemosensitivity to paclitaxel in pelvic lymph node metastasis.
- Source :
-
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2015 Mar; Vol. 70, pp. 217-26. Date of Electronic Publication: 2015 Feb 07. - Publication Year :
- 2015
-
Abstract
- Objective: MTDH/AEG-1 could act as an oncogene by regulating cellular transformation, proliferation, invasion, metastasis, and angiogenesis. This study aims to explore the mechanism by which MTDH/AEG-1 inhibits cancer growth and metastasis and enhances chemosensitivity.<br />Methods: Mouse model was established using orally immunized mice exposed to attenuated Salmonella containing vectors carrying full length MTDH/AEG-1 gene, and we were able to enhance the immune response and inhibit the growth and metastasis of prostate cancer through activation of cellular and humoral immunities and induction of CD8+ T cells. Immunohistochemistry and TUNEL assay, CD4+ and CD8+ T cell analysis by flow cytometry, HE staining, RT-PCR analysis, Western-blot analysis and quantitative polymerase chain reaction were performed.<br />Results: The MTDH/AEG-1 gene vaccine induced the anti-tumor function of cytotoxic T lymphocytes and CD8+ T cells and inhibited tumor growth and metastasis of prostate cancer. In the therapy model, the MTDH/AEG-1 gene vaccine significantly enhanced chemosensitivity to paclitaxel, inhibited tumor growth, promoted tumor cell apoptosis, and prolonged the survival time of tumor-bearing mice without any apparent side effects.<br />Conclusions: Our results demonstrated that MTDH/AEG-1-based DNA vaccines could used for the treatment of prostate cancer in terms of the inhibition of tumor growth, the lifespan of tumor-bearing animals. Combined with chemotherapy, MTDH/AEG-1-based DNA vaccines may produce highly favorable outcomes in the prevention and treatment of prostate cancer, suggesting the immune efficacy of MTDH/AEG-1-based DNA should be further analyzed in other cancers.<br /> (Copyright © 2015 Elsevier Masson SAS. All rights reserved.)
- Subjects :
- Administration, Oral
Animals
Cell Line, Tumor
Drug Resistance, Neoplasm drug effects
Lymphatic Metastasis
Male
Mice
Mice, Inbred C57BL
Pelvic Neoplasms mortality
Prostatic Neoplasms drug therapy
RNA-Binding Proteins
Survival Rate trends
Antineoplastic Agents, Phytogenic administration & dosage
Membrane Proteins administration & dosage
Paclitaxel administration & dosage
Pelvic Neoplasms prevention & control
Pelvic Neoplasms secondary
Vaccines, DNA administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1950-6007
- Volume :
- 70
- Database :
- MEDLINE
- Journal :
- Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
- Publication Type :
- Academic Journal
- Accession number :
- 25776504
- Full Text :
- https://doi.org/10.1016/j.biopha.2015.01.028