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Cepharanthine induces apoptosis through reactive oxygen species and mitochondrial dysfunction in human non-small-cell lung cancer cells.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2015 May 01; Vol. 460 (2), pp. 136-42. Date of Electronic Publication: 2015 Mar 05. - Publication Year :
- 2015
-
Abstract
- Cepharanthine is a medicinal plant-derived natural compound which possesses potent anti-cancer properties. However, there is little report about its effects on lung cancer cells. In this study, we investigated the effects of cepharanthine on the cell viability and apoptosis in human non-small-cell lung cancer H1299 and A549 cells. It was found that cepharanthine inhibited the growth of H1299 and A549 cells in a dose-dependent manner which was associated with the generation of reactive oxygen species(ROS) and the dissipation of mitochondrial membrane potential (Δψm). These effects were markedly abrogated when cells were pretreated with N-acetylcysteine (NAC), a specific ROS inhibitor, indicating that the apoptosis-inducing effect of cepharanthine in lung cancer cells was mediated by ROS. In addition, cepharanthine triggered apoptosis in non-small lung cancer cells via the upregulation of Bax, downregulation of Bcl-2 and significant activation of caspase-3 and PARP. These results provide the rationale for further research and preclinical investigation of cepharanthine's anti-tumor effect against human non-small-cell lung cancer.<br /> (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Subjects :
- Acetylcysteine pharmacology
Carcinoma, Non-Small-Cell Lung metabolism
Carcinoma, Non-Small-Cell Lung physiopathology
Cell Line, Tumor
Cell Proliferation drug effects
Humans
Lung Neoplasms metabolism
Lung Neoplasms physiopathology
Apoptosis drug effects
Benzylisoquinolines pharmacology
Carcinoma, Non-Small-Cell Lung pathology
Lung Neoplasms pathology
Membrane Potential, Mitochondrial drug effects
Reactive Oxygen Species metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 460
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 25747710
- Full Text :
- https://doi.org/10.1016/j.bbrc.2015.02.131