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Detection of DNA and poly-l-lysine using CVD graphene-channel FET biosensors.
- Source :
-
Nanotechnology [Nanotechnology] 2015 Mar 27; Vol. 26 (12), pp. 125502. Date of Electronic Publication: 2015 Mar 05. - Publication Year :
- 2015
-
Abstract
- A graphene channel field-effect biosensor is demonstrated for detecting the binding of double-stranded DNA and poly-l-lysine. Sensors consist of chemical vapor deposition graphene transferred using a clean, etchant-free transfer method. The presence of DNA and poly-l-lysine are detected by the conductance change of the graphene transistor. A readily measured shift in the Dirac voltage (the voltage at which the graphene's resistance peaks) is observed after the graphene channel is exposed to solutions containing DNA or poly-l-lysine. The 'Dirac voltage shift' is attributed to the binding/unbinding of charged molecules on the graphene surface. The polarity of the response changes to positive direction with poly-l-lysine and negative direction with DNA. This response results in detection limits of 8 pM for 48.5 kbp DNA and 11 pM for poly-l-lysine. The biosensors are easy to fabricate, reusable and are promising as sensors of a wide variety of charged biomolecules.
Details
- Language :
- English
- ISSN :
- 1361-6528
- Volume :
- 26
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Nanotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 25741743
- Full Text :
- https://doi.org/10.1088/0957-4484/26/12/125502