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Graphene oxide-assisted nucleic acids assays using conjugated polyelectrolytes-based fluorescent signal transduction.
- Source :
-
Analytical chemistry [Anal Chem] 2015 Apr 07; Vol. 87 (7), pp. 3877-83. Date of Electronic Publication: 2015 Mar 12. - Publication Year :
- 2015
-
Abstract
- In this work, we investigated the interactions between graphene oxide (GO) and conjugated polyelectrolytes (CPEs) with different backbone and side chain structures. By studying the mechanism of fluorescence quenching of CPEs by GO, we find that the charge and the molecular structure of CPEs play important roles for GO-CPEs interactions. Among them, electrostatic interaction, π-π interaction, and cation-π bonding are dominant driving forces. By using a cationic P2, we have developed a sensitive homogeneous sensor for DNA and RNA detection with a detection limit of 50 pM DNA and RNA, which increased the sensitivity by 40-fold as compared to GO-free CPE-based sensors. This GO-assisted CPE sensing strategy is also generic and shows a high potential for biosensor designs based on aptamers, proteins, peptides, and other biological probes.
Details
- Language :
- English
- ISSN :
- 1520-6882
- Volume :
- 87
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Analytical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 25738486
- Full Text :
- https://doi.org/10.1021/ac504658a