Back to Search
Start Over
Local heat activation of single myosins based on optical trapping of gold nanoparticles.
- Source :
-
Nano letters [Nano Lett] 2015 Apr 08; Vol. 15 (4), pp. 2456-61. Date of Electronic Publication: 2015 Mar 05. - Publication Year :
- 2015
-
Abstract
- Myosin is a mechano-enzyme that hydrolyzes ATP in order to move unidirectionally along actin filaments. Here we show by single molecule imaging that myosin V motion can be activated by local heat. We constructed a dark-field microscopy that included optical tweezers to monitor 80 nm gold nanoparticles (GNP) bound to single myosin V molecules with nanometer and submillisecond accuracy. We observed 34 nm processive steps along actin filaments like those seen when using 200 nm polystyrene beads (PB) but dwell times (ATPase activity) that were 4.5 times faster. Further, by using DNA nanotechnology (DNA origami) and myosin V as a nanometric thermometer, the temperature gradient surrounding optically trapped GNP could be estimated with nanometer accuracy. We propose our single molecule measurement system should advance quantitative analysis of the thermal control of biological and artificial systems like nanoscale thermal ratchet motors.
- Subjects :
- DNA ultrastructure
Gold chemistry
Materials Testing methods
Metal Nanoparticles chemistry
Molecular Motor Proteins chemistry
Molecular Motor Proteins ultrastructure
Molecular Probe Techniques
Myosins isolation & purification
Temperature
DNA chemistry
Heating methods
Molecular Imaging methods
Myosins chemistry
Optical Tweezers
Thermography methods
Subjects
Details
- Language :
- English
- ISSN :
- 1530-6992
- Volume :
- 15
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Nano letters
- Publication Type :
- Academic Journal
- Accession number :
- 25736894
- Full Text :
- https://doi.org/10.1021/nl5049059