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Local heat activation of single myosins based on optical trapping of gold nanoparticles.

Authors :
Iwaki M
Iwane AH
Ikezaki K
Yanagida T
Source :
Nano letters [Nano Lett] 2015 Apr 08; Vol. 15 (4), pp. 2456-61. Date of Electronic Publication: 2015 Mar 05.
Publication Year :
2015

Abstract

Myosin is a mechano-enzyme that hydrolyzes ATP in order to move unidirectionally along actin filaments. Here we show by single molecule imaging that myosin V motion can be activated by local heat. We constructed a dark-field microscopy that included optical tweezers to monitor 80 nm gold nanoparticles (GNP) bound to single myosin V molecules with nanometer and submillisecond accuracy. We observed 34 nm processive steps along actin filaments like those seen when using 200 nm polystyrene beads (PB) but dwell times (ATPase activity) that were 4.5 times faster. Further, by using DNA nanotechnology (DNA origami) and myosin V as a nanometric thermometer, the temperature gradient surrounding optically trapped GNP could be estimated with nanometer accuracy. We propose our single molecule measurement system should advance quantitative analysis of the thermal control of biological and artificial systems like nanoscale thermal ratchet motors.

Details

Language :
English
ISSN :
1530-6992
Volume :
15
Issue :
4
Database :
MEDLINE
Journal :
Nano letters
Publication Type :
Academic Journal
Accession number :
25736894
Full Text :
https://doi.org/10.1021/nl5049059