Back to Search
Start Over
A metabolomics approach to the identification of biomarkers of sugar-sweetened beverage intake.
- Source :
-
The American journal of clinical nutrition [Am J Clin Nutr] 2015 Mar; Vol. 101 (3), pp. 471-7. Date of Electronic Publication: 2015 Jan 21. - Publication Year :
- 2015
-
Abstract
- Background: The association between sugar-sweetened beverages (SSBs) and health risks remains controversial. To clarify proposed links, reliable and accurate dietary assessment methods of food intakes are essential.<br />Objective: The aim of this present work was to use a metabolomics approach to identify a panel of urinary biomarkers indicative of SSB consumption from a national food consumption survey and subsequently validate this panel in an acute intervention study.<br />Design: Heat map analysis was performed to identify correlations between ¹H nuclear magnetic resonance (NMR) spectral regions and SSB intakes in participants of the National Adult Nutrition Survey (n = 565). Metabolites were identified and receiver operating characteristic (ROC) analysis was performed to assess sensitivity and specificity of biomarkers. The panel of biomarkers was validated in an acute study (n = 10). A fasting first-void urine sample and postprandial samples (2, 4, 6 h) were collected after SSB consumption. After NMR spectroscopic profiling of the urine samples, multivariate data analysis was applied.<br />Results: A panel of 4 biomarkers-formate, citrulline, taurine, and isocitrate-were identified as markers of SSB intake. This panel of biomarkers had an area under the curve of 0.8 for ROC analysis and a sensitivity and specificity of 0.7 and 0.8, respectively. All 4 biomarkers were identified in the SSB sample. After acute consumption of an SSB drink, all 4 metabolites increased in the urine.<br />Conclusions: The present metabolomics-based strategy proved to be successful in the identification of SSB biomarkers. Future work will ascertain how to translate this panel of markers for use in nutrition epidemiology.<br /> (© 2015 American Society for Nutrition.)
- Subjects :
- Adolescent
Adult
Aged
Aged, 80 and over
Biomarkers metabolism
Biomarkers urine
Citrulline metabolism
Cohort Studies
Diet Surveys
Dietary Sucrose metabolism
Discriminant Analysis
Female
Follow-Up Studies
Formates metabolism
Humans
Ireland
Isocitrates metabolism
Magnetic Resonance Spectroscopy
Male
Middle Aged
Postprandial Period
Principal Component Analysis
Taurine metabolism
Young Adult
Beverages analysis
Citrulline urine
Dietary Sucrose administration & dosage
Formates urine
Isocitrates urine
Taurine urine
Up-Regulation
Subjects
Details
- Language :
- English
- ISSN :
- 1938-3207
- Volume :
- 101
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The American journal of clinical nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 25733631
- Full Text :
- https://doi.org/10.3945/ajcn.114.095604