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Evaluation of an antigen-capture EIA for the diagnosis of hepatitis E virus infection.
- Source :
-
Journal of viral hepatitis [J Viral Hepat] 2015 Nov; Vol. 22 (11), pp. 957-63. Date of Electronic Publication: 2015 Mar 03. - Publication Year :
- 2015
-
Abstract
- An enzyme immunoassay (EIA) has been developed for hepatitis E virus (HEV) antigen (HEV-Ag) detection and marketed in China. This study aimed to evaluate the sensitivity of the assay and assess the value of HEV-Ag detection in the diagnosis of HEV infection in comparison with HEV RNA detection. Using serial dilutions of a genotype 4 HEV strain, significant correlation was found between the EIA (S/CO) and HEV RNA (IU/mL) concentration in the range 10(3.5) to 10(0.5) IU/mL HEV RNA, the Pearson correlation coefficient r approached 0.97. The EIA detection limit was 54.6 IU/mL, compared to 24 IU/mL for HEV RNA using real-time RT-PCR. In clinical samples from hepatitis E patients, the HEV-Ag and HEV RNA positivity rates were 55.6% (65/117) and 60.7% (71/117) in sera and 76.7% (56/73) and 84.9% (62/73) in stools, and the concordance of these two markers was 77.8% in sera and 80.8% in stools. In serum samples, the HEV-Ag positivity rate and the concordance between HEV-Ag and HEV RNA were inversely proportional to the presence of anti-HEV antibody. The presence of anti-HEV IgG could reduce the S/CO of the HEV-Ag EIA. These results reveal a significant correlation between the detection of HEV-Ag and HEV RNA. The sensitivity of the HEV-Ag EIA was lower than real-time RT-PCR but could be higher than conventional nested RT-PCR. Therefore, the detection of HEV-Ag in serum and faeces is valuable for the diagnosis and prognosis of HEV infection in developing regions where real-time RT-PCR is not available.<br /> (© 2015 John Wiley & Sons Ltd.)
- Subjects :
- Animals
Blood virology
China
Feces virology
Humans
Molecular Sequence Data
RNA, Viral analysis
RNA, Viral genetics
Real-Time Polymerase Chain Reaction methods
Reverse Transcriptase Polymerase Chain Reaction methods
Sensitivity and Specificity
Sequence Analysis, DNA
Antigens, Viral analysis
Hepatitis E diagnosis
Hepatitis E virus immunology
Immunoenzyme Techniques methods
Subjects
Details
- Language :
- English
- ISSN :
- 1365-2893
- Volume :
- 22
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Journal of viral hepatitis
- Publication Type :
- Academic Journal
- Accession number :
- 25732029
- Full Text :
- https://doi.org/10.1111/jvh.12397