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The histone deacetylase Rpd3/Sin3/Ume6 complex represses an acetate-inducible isoform of VTH2 in fermenting budding yeast cells.
- Source :
-
FEBS letters [FEBS Lett] 2015 Apr 02; Vol. 589 (8), pp. 924-32. Date of Electronic Publication: 2015 Feb 26. - Publication Year :
- 2015
-
Abstract
- The tripartite Rpd3/Sin3/Ume6 complex represses meiotic isoforms during mitosis. We asked if it also controls starvation-induced isoforms. We report that VTH1/VTH2 encode acetate-inducible isoforms with extended 5'-regions overlapping antisense long non-coding RNAs. Rpd3 and Ume6 repress the long isoform of VTH2 during fermentation. Cells metabolising glucose contain Vth2, while the protein is undetectable in acetate and during sporulation. VTH2 is a useful model locus to study mechanisms implicating promoter directionality, lncRNA transcription and post-transcriptional control of gene expression via 5'-UTRs. Since mammalian genes encode transcript isoforms and Rpd3 is conserved, our findings are relevant for gene expression in higher eukaryotes.<br /> (Copyright © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- 5' Untranslated Regions genetics
Base Sequence
Enzyme Induction drug effects
Gene Expression Regulation, Fungal
Isoenzymes metabolism
Meiosis
Mutation
Promoter Regions, Genetic genetics
RNA, Untranslated genetics
Repressor Proteins metabolism
Saccharomyces cerevisiae enzymology
Saccharomyces cerevisiae Proteins genetics
Saccharomyces cerevisiae Proteins metabolism
Vesicular Transport Proteins genetics
Acetates pharmacology
Fermentation
Histone Deacetylases metabolism
Saccharomyces cerevisiae cytology
Saccharomyces cerevisiae metabolism
Saccharomyces cerevisiae Proteins biosynthesis
Vesicular Transport Proteins biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3468
- Volume :
- 589
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 25728275
- Full Text :
- https://doi.org/10.1016/j.febslet.2015.02.022