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Lipid mediators of inflammation as novel plasma biomarkers to identify patients with bacteremia.
- Source :
-
The Journal of infection [J Infect] 2015 May; Vol. 70 (5), pp. 433-44. Date of Electronic Publication: 2015 Feb 26. - Publication Year :
- 2015
-
Abstract
- Objectives: Rapid diagnostic tests for bacteremia are important for early treatment to improve clinical outcome. We sought to identify plasma biomarkers that can identify patients with bacteremia using an untargeted global metabolomic analysis.<br />Methods: Plasma metabolomic profiles were analyzed for 145 adult patients with (cases) and without (controls) bacteremia using ultra-high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF-MS). All metabolites were compared between cases and controls using a 2-tier filtering approach, and each metabolite underwent receiver operating characteristic (ROC) curve analysis. Individual metabolites that distinguish between cases and controls were characterized. Subgroup analysis was performed to identify metabolites with prognostic significance.<br />Results: After 2-tier filtering, 128 molecular features were identified to be potential biomarkers that could distinguish cases from controls. Five metabolites had an area under the ROC curve (AUC) of >0.8 in ROC curve analysis, including a sphingolipid, an acylcarnitine, a fatty acid ester, and 2 glycerophosphocholines. These metabolites could distinguish cases from controls in the unsupervised hierarchical clustering analysis. Subgroup analysis of bacteremic patients showed that the level of trans-2,3,4-trimethoxycinnamate was lower in fatal than non-fatal cases.<br />Conclusions: Plasma lipid mediators of inflammation can distinguish bacteremia cases from non-bacteremia controls. These biomarkers may be used as targets for rapid test in clinical practice.<br /> (Copyright © 2015 The British Infection Association. Published by Elsevier Ltd. All rights reserved.)
- Subjects :
- Adult
Aged
Aged, 80 and over
Bacteremia blood
Biomarkers blood
Carnitine analogs & derivatives
Carnitine blood
Chromatography, High Pressure Liquid
Fatty Acids blood
Female
Humans
Male
Metabolomics
Middle Aged
Prognosis
ROC Curve
Sphingolipids blood
Bacteremia diagnosis
Inflammation Mediators isolation & purification
Lipids blood
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2742
- Volume :
- 70
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The Journal of infection
- Publication Type :
- Academic Journal
- Accession number :
- 25727996
- Full Text :
- https://doi.org/10.1016/j.jinf.2015.02.011