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A role for matrix stiffness in the regulation of cardiac side population cell function.
- Source :
-
American journal of physiology. Heart and circulatory physiology [Am J Physiol Heart Circ Physiol] 2015 May 01; Vol. 308 (9), pp. H990-7. Date of Electronic Publication: 2015 Feb 27. - Publication Year :
- 2015
-
Abstract
- The mechanical properties of the local microenvironment may have important influence on the fate and function of adult tissue progenitor cells, altering the regenerative process. This is particularly critical following a myocardial infarction, in which the normal, compliant myocardial tissue is replaced with fibrotic, stiff scar tissue. In this study, we examined the effects of matrix stiffness on adult cardiac side population (CSP) progenitor cell behavior. Ovine and murine CSP cells were isolated and cultured on polydimethylsiloxane substrates, replicating the elastic moduli of normal and fibrotic myocardium. Proliferation capacity and cell cycling were increased in CSP cells cultured on the stiff substrate with an associated reduction in cardiomyogeneic differentiation and accelerated cell ageing. In addition, culture on stiff substrate stimulated upregulation of extracellular matrix and adhesion proteins gene expression in CSP cells. Collectively, we demonstrate that microenvironment properties, including matrix stiffness, play a critical role in regulating progenitor cell functions of endogenous resident CSP cells. Understanding the effects of the tissue microenvironment on resident cardiac progenitor cells is a critical step toward achieving functional cardiac regeneration.<br /> (Copyright © 2015 the American Physiological Society.)
- Subjects :
- Adult Stem Cells metabolism
Animals
Cell Adhesion
Cell Cycle
Cell Differentiation
Cell Proliferation
Cells, Cultured
Cellular Senescence
Coculture Techniques
Elastic Modulus
Male
Mice, Inbred C57BL
Myocytes, Cardiac metabolism
Phenotype
Sheep
Side-Population Cells metabolism
Time Factors
Adult Stem Cells physiology
Dimethylpolysiloxanes chemistry
Mechanotransduction, Cellular
Myocytes, Cardiac physiology
Side-Population Cells physiology
Stem Cell Niche
Subjects
Details
- Language :
- English
- ISSN :
- 1522-1539
- Volume :
- 308
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Heart and circulatory physiology
- Publication Type :
- Academic Journal
- Accession number :
- 25724498
- Full Text :
- https://doi.org/10.1152/ajpheart.00935.2014