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Homocitrate synthase expression and lysine content in fruiting body of different developmental stages in Flammulina velutipes.
- Source :
-
Current microbiology [Curr Microbiol] 2015 Jun; Vol. 70 (6), pp. 821-8. Date of Electronic Publication: 2015 Feb 28. - Publication Year :
- 2015
-
Abstract
- Homocitrate synthase (EC 2.3.3.14) regulates the first step of fungal lysine biosynthesis. The gene encoding homocitrate synthase was identified in whole genomic sequencing of Flammulina velutipes and contains seven introns. The homocitrate synthase gene of F. velutipes strain W23 (Fvhcs) is 1780 bp in length and encodes a 464 amino acid protein with a predicted molecular weight 50.7 kDa. Phylogenetic analysis of Fvhcs and other homocitrate synthase proteins from diverse fungi produced a topology congruent with the current best estimate of organismal phylogeny. Analysis of protein domains by InterProScan and a motif search found that Fvhcs gene encodes homocitrate synthase protein conserved across Agaricomycotina. In addition, we sequenced the transcriptome of different developmental stages and structures of the fruiting body to analyze the expression levels of the Fvhcs gene. The data showed a correlation between Fvhcs gene expression and lysine values in different developmental stages and structures of F. velutipes.
- Subjects :
- Cluster Analysis
DNA, Fungal chemistry
DNA, Fungal genetics
Flammulina genetics
Flammulina growth & development
Gene Expression Profiling
Genome, Fungal
Introns
Molecular Weight
Oxo-Acid-Lyases chemistry
Oxo-Acid-Lyases genetics
Phylogeny
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Flammulina chemistry
Flammulina enzymology
Gene Expression Regulation, Fungal
Lysine analysis
Oxo-Acid-Lyases biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1432-0991
- Volume :
- 70
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Current microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 25724343
- Full Text :
- https://doi.org/10.1007/s00284-015-0791-0