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Preparation and in vitro/in vivo evaluation of cyclosporin A-loaded nanodecorated ocular implants for subconjunctival application.
- Source :
-
Journal of pharmaceutical sciences [J Pharm Sci] 2015 May; Vol. 104 (5), pp. 1709-20. Date of Electronic Publication: 2015 Feb 25. - Publication Year :
- 2015
-
Abstract
- In terms of ocular drug delivery, biodegradable implant systems have several advantages including the ability to provide constant drug concentration at the target site, no necessity for surgical removal, and minimum systemic side effects. Cyclosporin A (CsA) is a neutral, hydrophobic, cyclic peptide of amino acids that frequently used for dry eye disease treatment. The aim of this study was to develop a nanoparticle-loaded implant system for sustained-release CsA delivery following subconjunctival implantation. Poly(lactide-co-glycolide) (85:15) or poly-ε-caprolactone (PCL) were used to prepare two different nanoparticle formulations. These nanoparticles loaded into PCL or poly(lactide-co-caprolactone) implant formulations were prepared by two different methods, which were molding and electrospinning. Size and zeta potential of nanoparticles were determined and the morphology of the formulations were investigated by scanning electron microscopy. CsA-loading efficiencies were calculated and the in vitro degradation and in vitro release studies were performed. MTT test was also performed using L929 fibroblast cells to evaluate the cytotoxicity of the formulations. PCL-PCL-NP-I formulation was implanted to Swiss Albino mice with induced dry eye syndrome to evaluate the efficacy. In vitro release studies showed that the release from the formulations continues between 30 and 60 days, and the cell viability was found to be 77.4%-99.0%. In vivo studies showed that healing is significantly faster in the presence of the selected implant formulation. Results indicated that nanodecorated implants are promising ocular carriers for controlled-release CsA application.<br /> (© 2015 Wiley Periodicals, Inc. and the American Pharmacists Association.)
- Subjects :
- Animals
Cyclosporine administration & dosage
Delayed-Action Preparations administration & dosage
Delayed-Action Preparations chemical synthesis
Drug Carriers administration & dosage
Drug Carriers chemical synthesis
Drug Evaluation methods
Drug Implants administration & dosage
Male
Mice
Nanoparticles administration & dosage
Polyesters administration & dosage
Polyesters chemical synthesis
Conjunctiva drug effects
Cyclosporine chemical synthesis
Drug Implants chemical synthesis
Nanoparticles chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1520-6017
- Volume :
- 104
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of pharmaceutical sciences
- Publication Type :
- Academic Journal
- Accession number :
- 25716582
- Full Text :
- https://doi.org/10.1002/jps.24385