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The conserved histone deacetylase Rpd3 and the DNA binding regulator Ume6 repress BOI1's meiotic transcript isoform during vegetative growth in Saccharomyces cerevisiae.
- Source :
-
Molecular microbiology [Mol Microbiol] 2015 May; Vol. 96 (4), pp. 861-74. Date of Electronic Publication: 2015 Mar 21. - Publication Year :
- 2015
-
Abstract
- BOI1 and BOI2 are paralogs important for the actin cytoskeleton and polar growth. BOI1 encodes a meiotic transcript isoform with an extended 5'-untranslated region predicted to impair protein translation. It is, however, unknown how the isoform is repressed during mitosis, and if Boi1 is present during sporulation. By interpreting microarray data from MATa cells, MATa/α cells, a starving MATα/α control, and a meiosis-impaired rrp6 mutant, we classified BOI1's extended isoform as early meiosis-specific. These results were confirmed by RNA-Sequencing, and extended by a 5'-RACE assay and Northern blotting, showing that meiotic cells induce the long isoform while the mitotic isoform remains detectable during meiosis. We provide evidence via motif predictions, an in vivo binding assay and genetic experiments that the Rpd3/Sin3/Ume6 histone deacetylase complex, which represses meiotic genes during mitosis, also prevents the induction of BOI1's 5'-extended isoform in mitosis by direct binding of Ume6 to its URS1 target. Finally, we find that Boi1 protein levels decline when cells switch from fermentation to respiration and sporulation. The histone deacetylase Rpd3 is conserved, and eukaryotic genes frequently encode transcripts with variable 5'-UTRs. Our findings are therefore relevant for regulatory mechanisms involved in the control of transcript isoforms in multi-cellular organisms.<br /> (© 2015 John Wiley & Sons Ltd.)
- Subjects :
- Base Sequence
Fermentation genetics
Histone Deacetylases genetics
Mitosis
Models, Molecular
Mutation
Protein Isoforms genetics
Repressor Proteins genetics
Saccharomyces cerevisiae growth & development
Saccharomyces cerevisiae metabolism
Tissue Array Analysis
Adaptor Proteins, Signal Transducing genetics
Adaptor Proteins, Signal Transducing metabolism
Gene Expression Regulation, Fungal
Histone Deacetylases metabolism
Meiosis genetics
Repressor Proteins metabolism
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae Proteins genetics
Saccharomyces cerevisiae Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1365-2958
- Volume :
- 96
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Molecular microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 25708805
- Full Text :
- https://doi.org/10.1111/mmi.12976