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Influenza A viruses of swine circulating in the United States during 2009-2014 are susceptible to neuraminidase inhibitors but show lineage-dependent resistance to adamantanes.
- Source :
-
Antiviral research [Antiviral Res] 2015 May; Vol. 117, pp. 10-9. Date of Electronic Publication: 2015 Feb 19. - Publication Year :
- 2015
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Abstract
- Antiviral drug susceptibility is one of the evaluation criteria of pandemic potential posed by an influenza virus. Influenza A viruses of swine (IAV-S) can play an important role in generating novel variants, yet limited information is available on the drug resistance profiles of IAV-S circulating in the U.S. Phenotypic analysis of the IAV-S isolated in the U.S. (2009-2011) (n=105) revealed normal inhibition by the neuraminidase (NA) inhibitors (NAIs) oseltamivir, zanamivir, and peramivir. Screening NA sequences from IAV-S collected in the U.S. (1930-2014) showed 0.03% (1/3396) sequences with clinically relevant H274Y-NA substitution. Phenotypic analysis of IAV-S isolated in the U.S. (2009-2011) confirmed amantadine resistance caused by the S31N-M2 and revealed an intermediate level of resistance caused by the I27T-M2. The majority (96.7%, 589/609) of IAV-S with the I27T-M2 in the influenza database were isolated from pigs in the U.S. The frequency of amantadine-resistant markers among IAV-S in the U.S. was high (71%), and their distribution was M-lineage dependent. All IAV-S of the Eurasian avian M lineage were amantadine-resistant and possessed either a single S31N-M2 substitution (78%, 585/747) or its combination with the V27A-M2 (22%, 162/747). The I27T-M2 substitution accounted for 43% (429/993) of amantadine resistance in classic swine M lineage. Phylogenetic analysis showed that both S31N-M2 and I27T-M2 emerged stochastically but appeared to be fixed in the U.S. IAV-S population. This study defines a drug-susceptibility profile, identifies the frequency of drug-resistant markers, and establishes a phylogenetic approach for continued antiviral-susceptibility monitoring of IAV-S in the U.S.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Subjects :
- Acids, Carbocyclic
Amino Acid Substitution
Animals
Base Sequence
Cyclopentanes pharmacology
Dogs
Drug Resistance, Viral genetics
Enzyme Inhibitors pharmacology
Genotype
Guanidines pharmacology
Influenza A Virus, H1N1 Subtype drug effects
Influenza A Virus, H1N1 Subtype genetics
Influenza A Virus, H1N2 Subtype drug effects
Influenza A Virus, H1N2 Subtype genetics
Influenza A Virus, H3N2 Subtype drug effects
Influenza A Virus, H3N2 Subtype genetics
Influenza A virus genetics
Madin Darby Canine Kidney Cells
Mutation, Missense
Orthomyxoviridae Infections drug therapy
Orthomyxoviridae Infections virology
Phenotype
Phylogeny
Swine
Time Factors
United States
Viral Proteins genetics
Zanamivir pharmacology
Adamantane pharmacology
Antiviral Agents pharmacology
Influenza A virus drug effects
Neuraminidase antagonists & inhibitors
Orthomyxoviridae Infections veterinary
Oseltamivir pharmacology
Swine Diseases virology
Subjects
Details
- Language :
- English
- ISSN :
- 1872-9096
- Volume :
- 117
- Database :
- MEDLINE
- Journal :
- Antiviral research
- Publication Type :
- Academic Journal
- Accession number :
- 25701593
- Full Text :
- https://doi.org/10.1016/j.antiviral.2015.02.004