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FRET study of G-quadruplex forming fluorescent oligonucleotide probes at the lipid monolayer interface.
- Source :
-
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2016 Jan 05; Vol. 152, pp. 614-21. Date of Electronic Publication: 2015 Feb 07. - Publication Year :
- 2016
-
Abstract
- Spectral properties and G-quadruplex folding ability of fluorescent oligonucleotide probes at the cationic dioctadecyldimethylammonium bromide (DODAB) monolayer interface are reported. Two oligonucleotides, a 19-mer bearing thrombin binding aptamer sequence and a 21-mer with human telomeric sequence, were end-labeled with fluorescent groups (FAM and TAMRA) to give FRET probes F19T and F21T, respectively. The probes exhibited abilities to fold into a quadruplex structure and to bind metal cations (Na(+) and K(+)). Fluorescence spectra of G-quadruplex FRET probes at the monolayer interface are reported for the first time. Investigations included film balance measurements (π-A isotherms) and fluorescence spectra recording using a fiber optic accessory interfaced with a spectrofluorimeter. The effect of the presence of DODAB monolayer, metal cations and the surface pressure of monolayer on spectral behavior of FRET probes were examined. Adsorption of probe at the cationic monolayer interface resulted in the FRET signal enhancement even in the absence of metal cations. Variation in the monolayer surface pressure exerted rather modest effect on the spectral properties of probes. The fluorescence energy transfer efficiency of monolayer adsorbed probes increased significantly in the presence of sodium or potassium ion in subphase, which indicated that the probes retained their cation binding properties when adsorbed at the monolayer interface.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Subjects :
- Base Sequence
Humans
Lipids chemistry
Potassium chemistry
Sodium chemistry
Surface Properties
Telomere chemistry
Aptamers, Nucleotide chemistry
Fluorescence Resonance Energy Transfer methods
Fluorescent Dyes chemistry
G-Quadruplexes
Oligonucleotide Probes chemistry
Quaternary Ammonium Compounds chemistry
Surface-Active Agents chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3557
- Volume :
- 152
- Database :
- MEDLINE
- Journal :
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
- Publication Type :
- Academic Journal
- Accession number :
- 25698056
- Full Text :
- https://doi.org/10.1016/j.saa.2015.01.102