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Detection of novel Chlamydiae and Legionellales from human nasal samples of healthy volunteers.
- Source :
-
Folia microbiologica [Folia Microbiol (Praha)] 2015 Jul; Vol. 60 (4), pp. 325-34. Date of Electronic Publication: 2015 Feb 20. - Publication Year :
- 2015
-
Abstract
- Chlamydiae are intracellular bacterial parasites of eukaryotes, ranging from amoebae to humans. They comprise many novel members and are investigated as emerging pathogens. Environmental studies highlighted similarities between the ecologies of chlamydiae and legionellae, both groups being important agents of respiratory infections. Herein, we analyzed nasal samples from healthy persons, searching for the presence of amoebae, chlamydiae and legionellae. From a total of 25 samples, we recovered by PCR eight samples positive to chlamydiae and six samples positive to legionellae. Among these samples, four were positive to both organisms. The sequencing of 16S rDNAs allowed to identify (i) among Chlamydiae: Parachlamydia acanthamoebae, Chlamydophila psittaci, Chlamydophila felis, and members of Rhabdochlamydiaceae, Simkaniaceae and E6 lineage and (ii) among Legionellaceae: Legionella longbeachae, Legionella bozemanii and Legionella impletisoli. Unexpectedly, we also recovered Diplorickettsia sp. Amoebae collected from nasal mucosae, Acanthamoeba and Vermamoeba, were endosymbiont-free, and chlamydiae revealed refractory to amoeba coculture. This study shows common exposure to chlamydiae and legionellae and suggests open air activities like gardening as a probable additional source of infection.
- Subjects :
- Amoeba classification
Amoeba genetics
Amoeba isolation & purification
Chlamydiales classification
Chlamydiales genetics
Cluster Analysis
DNA, Bacterial chemistry
DNA, Bacterial genetics
DNA, Protozoan chemistry
DNA, Protozoan genetics
DNA, Ribosomal chemistry
DNA, Ribosomal genetics
Healthy Volunteers
Humans
Legionellaceae classification
Legionellaceae genetics
Phylogeny
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Chlamydiales isolation & purification
Legionellaceae isolation & purification
Microbiota
Nasal Cavity microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1874-9356
- Volume :
- 60
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Folia microbiologica
- Publication Type :
- Academic Journal
- Accession number :
- 25697709
- Full Text :
- https://doi.org/10.1007/s12223-015-0378-y