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[Stress proteins in the cells of Porphyra purpurea (Rhodophyta) thallus].
- Source :
-
Tsitologiia [Tsitologiia] 2014; Vol. 56 (7), pp. 536-9. - Publication Year :
- 2014
-
Abstract
- Heat shock proteins have been revealed for the first time by the methods of Western blotting using alkaline phosphatase and ECL in the cells of Porphyra purpurea from Kattegat area of the Baltic Sea in normal and experimental stress conditions. It was demonstrated with application of monoclonal anti-Hsp70 antibodies that a slight band about 70 kDa is present constitutively at the film; additionally the polypeptide of about 40 kDa ("Hsp40") has been detected. After heat shock at 28 degrees C during 1 hr significant "expenditure" of Hsp70 was observed, as well as the pronounced induction of "Hsp40"; the induction was expressed especially strongly in 24 hr after the stress application.
- Subjects :
- Algal Proteins biosynthesis
Algal Proteins isolation & purification
Alkaline Phosphatase chemistry
Blotting, Western
Gene Expression
HSP40 Heat-Shock Proteins biosynthesis
HSP40 Heat-Shock Proteins isolation & purification
HSP70 Heat-Shock Proteins biosynthesis
HSP70 Heat-Shock Proteins isolation & purification
Hot Temperature
Luminescent Measurements
Porphyra metabolism
Stress, Physiological
Adaptation, Physiological genetics
Algal Proteins genetics
HSP40 Heat-Shock Proteins genetics
HSP70 Heat-Shock Proteins genetics
Porphyra genetics
Subjects
Details
- Language :
- Russian
- ISSN :
- 0041-3771
- Volume :
- 56
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Tsitologiia
- Publication Type :
- Academic Journal
- Accession number :
- 25696998