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Bio-mimetic mineralization potential of collagen hydrolysate obtained from chromium tanned leather waste.
- Source :
-
Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2015 Apr; Vol. 49, pp. 338-347. Date of Electronic Publication: 2015 Jan 08. - Publication Year :
- 2015
-
Abstract
- Hydroxyapatite (HA) ceramics serve as an alternative to autogenous-free bone grafting by virtue of their excellent biocompatibility. However, chemically synthesized HA lacks the strong load-bearing capacity as required by bone. The bio-mimetic growth of HA crystals on collagen surface provides a feasible solution for synthesizing bone substitutes with the desired properties. This study deals with the utilization of the collagen hydrolysate recovered from leather waste as a substrate for promoting HA crystal growth. Bio-mimetic growth of HA was induced by subjecting the hydrolysate to various mineralization conditions. Parameters that would have a direct effect on crystal growth were varied to determine the optimal conditions necessary. Maximum mineralization was achieved with a combination of 10mM of CaCl2, 5mM of Na2HPO4, 100mM of NaCl and 0.575% glutaraldehyde at a pH of 7.4. The metal-protein interactions leading to formation of HA were identified through Fourier-transform infrared (FTIR) spectroscopy and x-ray diffraction (XRD) studies. The crystal dimensions were determined to be in the nanoscale range by atomic force microscopy (AFM) and scanning electron microscopy (SEM). The size and crystallinity of bio-mimetically grown HA indicate that hydrolysate from leather waste can be used as an ideal alternative substrate for bone growth.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Subjects :
- Biocompatible Materials chemistry
Biomimetics methods
Bone Substitutes chemistry
Bone Transplantation methods
Bone and Bones chemistry
Crystallization methods
Durapatite chemistry
Microscopy, Electron, Scanning methods
Spectroscopy, Fourier Transform Infrared methods
X-Ray Diffraction methods
Chromium chemistry
Collagen chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1873-0191
- Volume :
- 49
- Database :
- MEDLINE
- Journal :
- Materials science & engineering. C, Materials for biological applications
- Publication Type :
- Academic Journal
- Accession number :
- 25686958
- Full Text :
- https://doi.org/10.1016/j.msec.2015.01.027