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Evaluation of different parameters in the extraction of incurred pesticides and environmental contaminants in fish.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2015 Jun 03; Vol. 63 (21), pp. 5163-8. Date of Electronic Publication: 2015 Feb 20. - Publication Year :
- 2015
-
Abstract
- Sample processing is often ignored during analytical method development and validation, but accurate results for real samples depend on all aspects of the analytical process. Also, validation is often conducted using only spiked samples, but extraction yields may be lower in incurred samples. In this study, different variables in extraction for incurred pesticides and environmental contaminants in fish were investigated. Among 207 analytes screened using low-pressure gas chromatography-tandem mass spectrometry, consisting of 150 pesticides, 15 polycyclic aromatic hydrocarbons (PAHs), 14 polychlorinated biphenyls (PCBs), 6 polybrominated diphenyl ethers (PBDEs), and 22 other flame retardants (FRs), 35 (16 pesticides, 9 PCBs, 5 PBDEs, and 5 PAHs) were identified for quantification in samples of salmon, croaker, and NIST Standard Reference Material 1947 (Lake Michigan Fish Tissue). Extraction efficiencies using different extraction devices (blending, vortexing, and vibrating) versus time, sample size, and sample/solvent ratio were determined. In comparison to blending results, use of a pulsed-vortexer for 1 min with 1/1 (g/mL) sample/acetonitrile ratio was generally sufficient to extract the incurred contaminants in the homogenized fish tissues. Conversely, extraction with a prototype vibration shaker often took 60 min to achieve 100% extraction efficiency. A main conclusion from this study is that accurate results for real samples can be obtained using batch extraction with a pulsed-vortexer in a simple and efficient method that achieves high sample throughput.
- Subjects :
- Analytic Sample Preparation Methods instrumentation
Animals
Environmental Pollution
Fishes
Flame Retardants analysis
Food Contamination analysis
Gas Chromatography-Mass Spectrometry
Pesticides chemistry
Water Pollutants, Chemical chemistry
Analytic Sample Preparation Methods methods
Flame Retardants isolation & purification
Pesticides isolation & purification
Seafood analysis
Water Pollutants, Chemical isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 63
- Issue :
- 21
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 25686151
- Full Text :
- https://doi.org/10.1021/jf506256q