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Immune responses of bison and efficacy after booster vaccination with Brucella abortus strain RB51.
- Source :
-
Clinical and vaccine immunology : CVI [Clin Vaccine Immunol] 2015 Apr; Vol. 22 (4), pp. 440-7. Date of Electronic Publication: 2015 Feb 11. - Publication Year :
- 2015
-
Abstract
- Thirty-one bison heifers were randomly assigned to receive saline or a single vaccination with 10(10) CFU of Brucella abortus strain RB51. Some vaccinated bison were randomly selected for booster vaccination with RB51 at 11 months after the initial vaccination. Mean antibody responses to RB51 were greater (P < 0.05) in vaccinated bison after initial and booster vaccination than in nonvaccinated bison. The proliferative responses by peripheral blood mononuclear cells (PBMC) from the vaccinated bison were greater (P < 0.05) than those in the nonvaccinated bison at 16 and 24 weeks after the initial vaccination but not after the booster vaccination. The relative gene expression of gamma interferon (IFN-γ) was increased (P < 0.05) in the RB51-vaccinated bison at 8, 16, and 24 weeks after the initial vaccination and at 8 weeks after the booster vaccination. The vaccinated bison had greater (P < 0.05) in vitro production of IFN-γ at all sampling times, greater interleukin-1β (IL-1β) production in various samplings after the initial and booster vaccinations, and greater IL-6 production at one sampling time after the booster vaccination. Between 170 and 180 days of gestation, the bison were intraconjunctivally challenged with approximately 1 × 10(7) CFU of B. abortus strain 2308. The incidences of abortion and infection were greater (P < 0.05) in the nonvaccinated bison after experimental challenge than in the bison receiving either vaccination treatment. Booster-vaccinated, but not single-vaccinated bison, had a reduced (P < 0.05) incidence of infection in fetal tissues and maternal tissues compared to that in the controls. Compared to the nonvaccinated bison, both vaccination treatments lowered the colonization (measured as the CFU/g of tissue) of Brucella organisms in all tissues, except in retropharyngeal and supramammary lymph nodes. Our study suggests that RB51 booster vaccination is an effective vaccination strategy for enhancing herd immunity against brucellosis in bison.<br /> (Copyright © 2015, American Society for Microbiology. All Rights Reserved.)
- Subjects :
- Animal Structures microbiology
Animals
Bacterial Load
Brucella Vaccine administration & dosage
Brucellosis prevention & control
Cell Proliferation
Gene Expression Profiling
Interferon-gamma biosynthesis
Interferon-gamma metabolism
Interleukin-6 metabolism
Random Allocation
Treatment Outcome
Antibodies, Bacterial blood
Bison
Brucella Vaccine immunology
Brucella abortus immunology
Brucellosis veterinary
Immunization, Secondary methods
Leukocytes, Mononuclear immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1556-679X
- Volume :
- 22
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Clinical and vaccine immunology : CVI
- Publication Type :
- Academic Journal
- Accession number :
- 25673305
- Full Text :
- https://doi.org/10.1128/CVI.00746-14