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Imaging approaches to measuring lysosomal calcium.
- Source :
-
Methods in cell biology [Methods Cell Biol] 2015; Vol. 126, pp. 159-95. Date of Electronic Publication: 2015 Jan 19. - Publication Year :
- 2015
-
Abstract
- Endolysosomes are emerging as key players that generate as well as respond to intracellular Ca(2+) signals. The role of Ca(2+) in modulating acidic organelle function has long been recognized, but it is now emerging that acidic organelles also act as intracellular Ca(2+) stores; they actively sequester Ca(2+) in their lumina and release it to the cytosol upon activation of endolysosomal Ca(2+) channels. This local Ca(2+) signal is crucial for endolysosomal function and/or global Ca(2+) signaling. Importantly, defects in endolysosomal Ca(2+) are associated with disease. This chapter discusses several complimentary approaches to monitor endolysosomal Ca(2+), with particular emphasis on the inherent pitfalls that can plague the unwary.<br /> (Copyright © 2015 Elsevier Inc. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 0091-679X
- Volume :
- 126
- Database :
- MEDLINE
- Journal :
- Methods in cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 25665446
- Full Text :
- https://doi.org/10.1016/bs.mcb.2014.10.031