Back to Search
Start Over
Stepwise changes in viable but nonculturable Vibrio cholerae cells.
- Source :
-
Microbiology and immunology [Microbiol Immunol] 2015 May; Vol. 59 (5), pp. 305-10. - Publication Year :
- 2015
-
Abstract
- Many bacterial species are known to become viable but nonculturable (VBNC) under conditions that are unsuitable for growth. In this study, the requirements for resuscitation of VBNC-state Vibrio cholerae cells were found to change over time. Although VBNC cells could initially be converted to culturable by treatment with catalase or HT-29 cell extract, they subsequently entered a state that was not convertible to culturable by these factors. However, fluorescence microscopy revealed the presence of live cells in this state, from which VBNC cells were resuscitated by co-cultivation with HT-29 human colon adenocarcinoma cells. Ultimately, all cells entered a state from which they could not be resuscitated, even by co-cultivation with HT-29. These characteristic changes in VBNC-state cells were a common feature of strains in both V. cholerae O1 and O139 serogroups. Thus, the VBNC state of V. cholerae is not a single property but continues to change over time.<br /> (© 2015 The Societies and Wiley Publishing Asia Pty Ltd.)
- Subjects :
- Bacteriological Techniques methods
Cell Line, Tumor
Coculture Techniques methods
Epithelial Cells microbiology
Epithelial Cells physiology
Humans
Vibrio cholerae O1 growth & development
Vibrio cholerae O139 growth & development
Microbial Viability
Vibrio cholerae O1 physiology
Vibrio cholerae O139 physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1348-0421
- Volume :
- 59
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Microbiology and immunology
- Publication Type :
- Academic Journal
- Accession number :
- 25664673
- Full Text :
- https://doi.org/10.1111/1348-0421.12246