Back to Search
Start Over
Functional analysis of porcine reproductive and respiratory syndrome virus N-glycans in infection of permissive cells.
- Source :
-
Virology [Virology] 2015 Mar; Vol. 477, pp. 82-88. Date of Electronic Publication: 2015 Feb 06. - Publication Year :
- 2015
-
Abstract
- The role of envelope protein-linked N-glycans in porcine reproductive and respiratory syndrome virus (PRRSV) infection of permissive cells was examined. N-acetylglucosamine (GlcNAc) and N-acetyllactosamine (LacNAc) oligomer-specific lectins bound to PRRSV and blocked virus attachment, resulting in reduced viral infection. However, addition of GlcNAc oligomers and LacNAc to cell culture together with PRRSV did not block infection. Removal or alteration of envelope protein-linked N-glycans also did not affect virus infection, indicating that PRRSV N-glycans are not required for virus infection. These findings show that steric hindrance of glycans on the PRRSV envelope by lectins or, presumably, other space-filling molecules, may interfere nonspecifically with infection by blocking protein interactions with cell surface receptors. Glycans themselves appear not to be required for infection of permissive cells, but may have important roles in avoidance of host immunity and in protein structure, intracellular virion growth and assembly.<br /> (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Cells, Cultured
Lectins metabolism
Swine
Virus Attachment drug effects
Polysaccharides metabolism
Porcine respiratory and reproductive syndrome virus chemistry
Porcine respiratory and reproductive syndrome virus physiology
Viral Envelope Proteins chemistry
Viral Envelope Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0341
- Volume :
- 477
- Database :
- MEDLINE
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 25662311
- Full Text :
- https://doi.org/10.1016/j.virol.2015.01.005