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Down-regulation of monocarboxylate transporter 1 (MCT1) gene expression in the colon of piglets is linked to bacterial protein fermentation and pro-inflammatory cytokine-mediated signalling.
- Source :
-
The British journal of nutrition [Br J Nutr] 2015 Feb 28; Vol. 113 (4), pp. 610-7. Date of Electronic Publication: 2015 Feb 06. - Publication Year :
- 2015
-
Abstract
- The present study investigated the influence of bacterial metabolites on monocarboxylate transporter 1 (MCT1) expression in pigs using in vivo, ex vivo and in vitro approaches. Piglets (n 24) were fed high-protein (26 %) or low-protein (18 %) diets with or without fermentable carbohydrates. Colonic digesta samples were analysed for a broad range of bacterial metabolites. The expression of MCT1, TNF-α, interferon γ (IFN-γ) and IL-8 was determined in colonic tissue. The expression of MCT1 was lower and of TNF-α and IL-8 was higher with high-protein diets (P< 0·05). MCT1 expression was positively correlated with l-lactate, whereas negatively correlated with NH₃ and putrescine (P< 0·05). The expression of IL-8 and TNF-α was negatively correlated with l-lactate and positively correlated with NH₃ and putrescine, whereas the expression of IFN-γ was positively correlated with histamine and 4-ethylphenol (P< 0·05). Subsequently, porcine colonic tissue and Caco-2 cells were incubated with Na-butyrate, NH₄Cl or TNF-α as selected bacterial metabolites or mediators of inflammation. Colonic MCT1 expression was higher after incubation with Na-butyrate (P< 0·05) and lower after incubation with NH₄Cl or TNF-α (P< 0·05). Incubation of Caco-2 cells with increasing concentrations of these metabolites confirmed the up-regulation of MCT1 expression by Na-butyrate (linear, P< 0·05) and down-regulation by TNF-α and NH₄Cl (linear, P< 0·05). The high-protein diet decreased the expression of MCT1 in the colon of pigs, which appears to be linked to NH₃- and TNF-α-mediated signalling.
- Subjects :
- Animals
Caco-2 Cells
Colon growth & development
Colon immunology
Colon microbiology
Crosses, Genetic
Dietary Carbohydrates adverse effects
Dietary Carbohydrates metabolism
Dietary Proteins adverse effects
Energy Intake
Female
Fermentation
Humans
Intestinal Mucosa growth & development
Intestinal Mucosa immunology
Intestinal Mucosa microbiology
Male
Monocarboxylic Acid Transporters genetics
Random Allocation
Sus scrofa
Symporters genetics
Weaning
Weight Gain
Colon metabolism
Cytokines metabolism
Dietary Proteins metabolism
Gene Expression Regulation, Developmental
Intestinal Mucosa metabolism
Monocarboxylic Acid Transporters metabolism
Signal Transduction
Symporters metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1475-2662
- Volume :
- 113
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- The British journal of nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 25656974
- Full Text :
- https://doi.org/10.1017/S0007114514004231