A fluorescent Arg-Gly-Asp (RGD) peptide-naphthalenediimide (NDI) conjugate for imaging integrin α(v)β(3) in vitro.

Authors :: Hu Z
Arrowsmith RL
Tyson JA
Mirabello V
Ge H
Eggleston IM
Botchway SW
Pantos GD
Pascu SI
Source :: Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2015 Apr 25; Vol. 51 (32), pp. 6901-4. Date of Electronic Publication: 2015 Feb 03.
Publication Year :: 2015
Abstract: We have developed a fluorescent peptide conjugate (TrpNDIRGDfK) based on the coupling of cyclo(RGDfK) to a new tryptophan-tagged amino acid naphthalenediimide (TrpNDI). Confocal fluorescence microscopy coupled with fluorescence lifetime imaging (FLIM) mapping, single and two-photon fluorescence excitation, lifetime components and corresponding decay profiles were used as parameters able to investigate qualitatively the cellular behavior regarding the molecular environment and biolocalisation of TrpNDI and TrpNDI-RGDfK in cancer cells.

Subjects :: Animals
Cell Line, Tumor
Humans
Integrin alphaVbeta3 chemistry
Models, Molecular
Molecular Conformation
Fluorescent Dyes chemistry
Imides chemistry
Integrin alphaVbeta3 metabolism
Molecular Imaging methods
Naphthalenes chemistry
Oligopeptides chemistry

Full Text Access

Tools