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Biotransformation of zearalenone and zearalenols to their major glucuronide metabolites reduces estrogenic activity.
- Source :
-
Toxicology in vitro : an international journal published in association with BIBRA [Toxicol In Vitro] 2015 Apr; Vol. 29 (3), pp. 575-81. Date of Electronic Publication: 2015 Jan 30. - Publication Year :
- 2015
-
Abstract
- Zearalenone (ZEN) is a mycotoxin produced by Fusarium fungi. Once ingested, ZEN may be absorbed and metabolised to α- and β-zearalenol (α-ZOL, β-ZOL), and to a lesser extent α- and β-zearalanol (α-ZAL, β-ZAL). Further biotransformation to glucuronide conjugates also occurs to facilitate the elimination of these toxins from the body. Unlike ZEN and its metabolites, information regarding the estrogenic activity of these glucuronide conjugates in various tissues is lacking. ZEN-14-O-glucuronide, α-ZOL-14-O-glucuronide, α-ZOL-7-O-glucuronide, β-ZOL-14-O-glucuronide and β-ZOL-16-O-glucuronide, previously obtained as the major products from preparative enzymatic synthesis, were investigated for their potential to cause endocrine disruption through interference with estrogen receptor transcriptional activity. All five glucuronide conjugates showed a very weak agonist response in an estrogen responsive reporter gene assay (RGA), with activity ranging from 0.0001% to 0.01% of that of 17β-estradiol, and also less than that of ZEN, α-ZOL and β-ZOL which have previously shown estrogenic potencies of the order 17β-estradiol>α-ZOL>ZEN>β-ZOL. Confirmatory mass spectrometry revealed that any activity observed was likely a result of minor deconjugation of the glucuronide moiety. This study confirms that formation of ZEN and ZOL glucuronides is a detoxification reaction with regard to estrogenicity, serving as a potential host defence mechanism against ZEN-induced estrogenic activity.<br /> (Copyright © 2015 Elsevier Ltd. All rights reserved.)
- Subjects :
- Biotransformation
Cell Survival drug effects
Genes, Reporter drug effects
Glucuronides metabolism
Humans
Zeranol metabolism
Zeranol toxicity
Estrogens, Non-Steroidal metabolism
Estrogens, Non-Steroidal toxicity
Mycotoxins metabolism
Mycotoxins toxicity
Zearalenone metabolism
Zearalenone toxicity
Zeranol analogs & derivatives
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3177
- Volume :
- 29
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Toxicology in vitro : an international journal published in association with BIBRA
- Publication Type :
- Academic Journal
- Accession number :
- 25645597
- Full Text :
- https://doi.org/10.1016/j.tiv.2015.01.006