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Cell-autonomous sex differences in gene expression in chicken bone marrow-derived macrophages.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2015 Mar 01; Vol. 194 (5), pp. 2338-44. Date of Electronic Publication: 2015 Jan 30. - Publication Year :
- 2015
-
Abstract
- We have identified differences in gene expression in macrophages grown from the bone marrow of male and female chickens in recombinant chicken M-CSF (CSF1). Cells were profiled with or without treatment with bacterial LPS for 24 h. Approximately 600 transcripts were induced by prolonged LPS stimulation to an equal extent in the male and female macrophages. Many transcripts encoded on the Z chromosome were expressed ∼1.6-fold higher in males, reflecting a lack of dosage compensation in the homogametic sex. A smaller set of W chromosome-specific genes was expressed only in females. LPS signaling in mammals is associated with induction of type 1 IFN-responsive genes. Unexpectedly, because IFNs are encoded on the Z chromosome of chickens, unstimulated macrophages from the female birds expressed a set of known IFN-inducible genes at much higher levels than male cells under the same conditions. To confirm that these differences were not the consequence of the actions of gonadal hormones, we induced gonadal sex reversal to alter the hormonal environment of the developing chick and analyzed macrophages cultured from male, female, and female sex-reversed embryos. Gonadal sex reversal did not alter the sexually dimorphic expression of either sex-linked or IFN-responsive genes. We suggest that female birds compensate for the reduced dose of inducible IFN with a higher basal set point of IFN-responsive genes.<br /> (Copyright © 2015 The Authors.)
- Subjects :
- Animals
Aromatase Inhibitors pharmacology
Avian Proteins genetics
Cells, Cultured
Chick Embryo
Chickens genetics
Dosage Compensation, Genetic
Fadrozole pharmacology
Female
Gene Expression
Gene Expression Profiling
Gonads drug effects
Interferon-alpha genetics
Interferon-alpha immunology
Interferon-beta genetics
Interferon-beta immunology
Lipopolysaccharides pharmacology
Macrophage Colony-Stimulating Factor pharmacology
Macrophages cytology
Macrophages drug effects
Male
RNA, Messenger genetics
Sex Characteristics
Avian Proteins immunology
Chickens immunology
Gonads immunology
Macrophages immunology
RNA, Messenger immunology
Sex Chromosomes immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1550-6606
- Volume :
- 194
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 25637020
- Full Text :
- https://doi.org/10.4049/jimmunol.1401982