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Morphological and proliferative studies on ex vivo cultured human anterior lens epithelial cells - relevance to capsular opacification.
- Source :
-
Acta ophthalmologica [Acta Ophthalmol] 2015 Sep; Vol. 93 (6), pp. e499-506. Date of Electronic Publication: 2015 Jan 28. - Publication Year :
- 2015
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Abstract
- Purpose: To determine the structural characteristics of lens epithelial cells (LECs) found on the anterior portion of the lens capsule and their pluripotency, proliferating and migrating potential when grown ex vivo with relevance to posterior capsular opacification (PCO) after cataract surgery.<br />Methods: The explants of anterior portion of the lens capsule consisting of monolayer of LECs were obtained from uneventful cataract surgery and were cultivated under adherent conditions. The size and shape of the outgrowing cells were recorded by scanning electron microscopy (SEM), while their migration and proliferation potential were followed using light microscopy. Positivity for proliferation (Ki-67)- and pluripotency (Sox2)-specific markers were tested by immunofluorescent staining.<br />Results: The proliferation and migration of anterior portion of the lens capsule's LECs filling up the denuded and reverse side regions of the lens capsule as well as their growth on glass culture surfaces could be followed by light microscopy and SEM, while the distribution of LECs and their morphology could be analysed in detail by SEM. The expression of Ki-67 and Sox2 in LECs growing adherently on human anterior portion of the lens capsule could also be detected.<br />Conclusions: Classic light microscopy and SEM can be used to show that human anterior portion of the lens capsule harbours LECs that can proliferate and migrate, suggesting their pluripotency or putative stem cell nature. Similarly, morphological techniques can be used to study PCO and the effect different drugs or physical treatments have against PCO development.<br /> (© 2015 Acta Ophthalmologica Scandinavica Foundation. Published by John Wiley & Sons Ltd.)
- Subjects :
- Aged
Biomarkers metabolism
Cataract Extraction
Cells, Cultured
Fluorescent Antibody Technique, Indirect
Humans
Ki-67 Antigen metabolism
Microscopy
Microscopy, Electron, Scanning
Middle Aged
Pluripotent Stem Cells cytology
SOXB1 Transcription Factors metabolism
Anterior Capsule of the Lens ultrastructure
Capsule Opacification prevention & control
Cell Movement physiology
Cell Proliferation physiology
Epithelial Cells ultrastructure
Lens, Crystalline ultrastructure
Subjects
Details
- Language :
- English
- ISSN :
- 1755-3768
- Volume :
- 93
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Acta ophthalmologica
- Publication Type :
- Academic Journal
- Accession number :
- 25631167
- Full Text :
- https://doi.org/10.1111/aos.12655