Ppp2ca knockout in mice spermatogenesis.

Authors :: Pan X
Chen X
Tong X
Tang C
Li J
Source :: Reproduction (Cambridge, England) [Reproduction] 2015 Apr; Vol. 149 (4), pp. 385-91. Date of Electronic Publication: 2015 Jan 27.
Publication Year :: 2015
Abstract: Protein phosphatase 2A (PP2A) is a ubiquitous serine/threonine phosphatase involved in meiosis, mitosis, sperm capacitation, and apoptosis. Abberant activity of PP2A has been associated with a number of diseases. The homolog PPP2CA and PPP2CB can each function as the phosphatase catalytic subunit generally referred to as PP2AC. We generated a Ppp2ca conditional knockout (CKO) in C57BL/6J mice. Exon 2 of Ppp2ca was knocked out in a spatial or temporal-specific manner in primordial germ cells at E12.5. This Ppp2ca-null mutation caused infertility in male C57BL/6J mice. These CKO mice provide a powerful tool to study the mechanisms of Ppp2ca in development and disease.<br /> (© 2015 Society for Reproduction and Fertility.)